Centre National De La Recherche Scientifique

Université Technologie De Compiègne-utc

Ecole Normale Superieure De Lyon

Institut National De L'environment Industriel Et Des Risques

Smrc Automotive Holdings Netherlands B.v.

Institut National De L' Environnement Industriel Et Des Risques

Eric Leclerc

Patrick Paullier

Cécile Legallais

Régis Baudoin

Cecile Legallais

Regis Baudoin

Marc-Emmanuel Dumas

Anne-Virginie Salsac

Dominique Barthes-Biesel

Thi Xuan Chu

Laetitia Shintu

Pierre Toulhoat

Céline Brochot

Franck Merlier

Jean-Matthieu Prot

Aissa Ould Dris

Alain Choquet

Aïssa Ould Dris

Celine Brochot

The present invention relates to a unit () comprising a microsystem () and at least one set of interface connections () for the microsystem (); the microsystem () comprising a bottom plate () bearing the impression of at least one microstructured dynamic cell culture chamber (), and a top plate (), characterized in that the at least one microstructured chamber () is fluidically connected at inlet and/or at outlet by a set of connections () inserted removably into a hole in the upper plate (). The present invention also relates to a dynamic cell culture system to this effect.

Multi-reactor unit for dynamic cell culture

The invention concerns a facility for coupling a bioreactor for culturing bio-organisms, in dynamic mode, with at least one device for physicochemically analyzing a fluid from this bioreactor or collecting samples of this fluid, the device being equipped with a pump and an injector, the bioreactor being equipped with means for supplying culture medium and xenobiotic(s). The facility is remarkable in that the bioreactor is connected to a closed perfusion loop provided with a pump, fitted with a sampling loop and in that the facility comprises:

Facility for coupling a bioreactor with a device for physicochemically analysing or collecting samples

The present invention relates to a method for producing microcapsules having a calibrated diameter and at least one parameter characteristic of the mechanical properties of an equally calibrated microcapsule, characterized in that the method includes the steps of: producing microcapsules by means of injection into a microsystem () of three fluids (f, f, f); deforming the microcapsules by means generating a flow within a capillary tube (); observing and acquiring the outline of at least one deformed microcapsule observed by an optical acquisition means (); comparing the acquired outline with outlines from a collection of reference microcapsule outlines; determining the diameter and said at least one parameter characteristic of the mechanical properties of the observed microcapsule; and modifying the flow rates (Q, Q, and/or Q) of the fluids (f, f, f). The present invention also relates to a system for producing calibrated microcapsules.

Method and system for producing calibrated microcapsules

The present invention relates to a method of toxicological evaluation of a candidate substance on at least one tissue or organ, characterized in that it comprises the steps of: (a) obtaining a bioartificial tissue or organ by simulation or modelling of the metabolic activity of said tissue or organ by at least one bioreactor; (b) exposure of said bioartificial tissue or organ to said substance; (c) observation of the metabolic response of the bioartificial tissue or organ and acquisition without a priori of an associated multidimensional data set; (d) identification by means of a method of multivariate statistical analysis of the components of the multidimensional data set which are quantitatively correlated with predetermined variables; (e) generation of a predictive model on the basis of the components of the data set that are actually retained; (f) testing of the predictive nature of said model by at least one statistical method of estimating reliability; (g) identification of the metabolic response of the bioartificial tissue or organ in the form of biomarkers associated with the components of the data set that are adopted for the model. The present invention also relates to a method of toxicological screening and to a system for this purpose.

Method of toxicological evaluation, method of toxicological screening and associated system

Growing community of inventors

Margny les Compiegne, France

Eric Leclerc