Applied Biosystems, LLC

Qiagen Gmbh

Qiagen Sciences, LLC

Eric Lader

Jie Kang

Alexander Azzawi

Peter Hahn

Wolfgang Bielke

The present invention pertains to the use of at least one abasic modification within the first 8 nucleotide positions of the 5' region of the antisense strand of a small interfering nucleic acid (siNA) molecule for reducing off-target effects. Provided are suitable modified siNAs, compositions and methods for producing respective siNAs, as well as kits comprising respective siNAs.

Modified siNA

The invention pertains to a method for performing an expression modulating analysis or assay, wherein an apoptosis inducing expression modulating compound targeting a repetitive element present in the untranslated region, in particular the 3' UTRs of protein coding gene transcripts is introduced into cells to induce apoptosis in the cells as a positive control. Also provided are suitable kits and compositions.

Apoptosis inducing positive control for expression modulation experiments

This specification relates to the field of molecular biology and provides novel methods and reagents for preserving and protecting the ribonucleic acid (RNA) content of samples from degradation prior to RNA isolation. This preservation may be accomplished without ultra-low temperature storage or disruption of the tissue.

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Boyds, MD, United States of America

Eric Lader