Ventana Medical Systems, Inc.

Eric Kaldjian

Noah Theiss

Bharathi Vennapusa

Harry James Hnatyszyn

Erik Olson

Jacquelyn Smith

Alton Yates

Disclosed herein are methods and systems for detecting CD3 and CD16 in the same sample of a colorectal, head & neck, or lung tumor using immunohistochemical methods using primary antibodies from the same host species and secondary antibodies immunoreactive with antibodies of the host species of the primary antibodies. Methods to denature and block the first primary antibody contacted with the sample are provided.

Multiples multiplex immunohistochemical assay using primary antibodies of the same host species

Disclosed herein are methods and systems for detecting two different epitopes in the same sample using immunohistochemical methods using primary antibodies from the same host species and secondary antibodies immunoreactive with antibodies of the host species of the primary antibodies. Methods to denature and block the first primary antibody contacted with the sample are provided.

Multiplex immunohistochemical assay using primary antibodies of the same host species

The present application provides methods and systems for detecting both CD3 and CD16 in a sample using immunohistochemical methods, for example when the primary antibodies for CD3 and CD16 are from the same species (e.g., both rabbit antibodies). Methods to denature and block the first primary antibody contacted with the sample are provided.

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Ann Arbor, MI, United States of America

Eric Kaldjian