Chr. Hansen A/s

Bioteknologisk Institut

Chr. Hanse A/s

Chr Hansen's Laboratorium Danmark A/s

Bioneer A/s

Eric Johansen

Kim Ib Soerensen

Annette Kibenich

Dan Nilsson

Mirjana Curic-Bawden

Mette Pia Junge

Egon Bech Hansen

Patrick Derkx

Mette Dines Cantor

Birgitte Stuer-Lauridsen

Beatrice Nielsen

Francedillaoise Dickely

Hans Israelsen

Astrid Vrang

Soeren Michael Madsen

Per Stro Slashedman

Kim Sorensen

Rasmus Larsen

Soren Michael Madsen

The dairy industry today faces a problem of providing an alternative to adding sweeteners to fermented milk products in order to achieve the desired sweet taste without the added calories. Furthermore, it would be highly advantageous to establish a method for reducing lactose in fermented milk products to a level which is acceptable for lactose-intolerant consumers. The above problems have been solved by providing mutantstrains and mutantsubsp.strains that excrete glucose to the milk when the milk is inoculated and fermented with suchstrains andsubsp,strains. Thus, the present invention relates to strains ofandsubsp,which secrete glucose to the milk substrate during fermentation, as well as to mixed cultures comprising thestrains and thesubsp,strains, starter cultures comprising the strains and dairy products manufactured with the cultures. The present method also relates to use of the strains for decreasing the lactose content of a fermented food product and for boosting growth of the probiotic BB-12®.

Use of lactic acid bacteria for preparing fermented food products with increased natural sweetness

The present invention relates to mutants of lactic acid bacteria which are resistant to the antibiotic ampicillin and which were found to give an increased texture when grown in milk while maintaining the other growth properties of the parent strain. The present invention, furthermore, relates to compositions comprising such mutants, and to dairy products fermented with the lactic acid bacteria resistant to ampicillin.

Ampicillin resistant texturizing lactic acid bacteria strains

Described are mutantstrains and mutantsubsp.strains that excrete glucose into milk when the milk is inoculated and fermented with such strains. Methods for obtaining such strains without genetic modification are described, including methods for obtaining suchstrains by selecting and isolating from a pool ofstrains derived from a galactose-fermentingmother strain a pool ofstrains which are resistant to 2-deoxyglucose, and selecting and isolating from the pool ofstrains which are resistant to 2-deoxyglucose astrain which exhibits a growth rate in M17 medium+2% galactose higher than its growth rate in M17 medium+2% glucose. The strains are useful for decreasing the lactose content of a fermented food product and for boosting growth of the probiotic BB-12&#xae;.

The dairy industry today faces a problem of providing an alternative to adding sweeteners to fermented milk products in order to achieve the desired sweet taste without the added calories. Furthermore, it would be highly advantageous to establish a method for reducing lactose in fermented milk products to a level which is acceptable for lactose-intolerant consumers. The above problems have been solved by providing mutantstrains and mutantsubsp.strains that excrete glucose to the milk when the milk is inoculated and fermented with suchstrains andsubsp,strains. Thus, the present invention relates to strains ofandsubsp,which secrete glucose to the milk substrate during fermentation, as well as to mixed cultures comprising thestrains and thesubsp,strains, starter cultures comprising the strains and dairy products manufactured with the cultures. The present method also relates to use of the strains for decreasing the lactose content of a fermented food product and for boosting growth of the probiotic BB-12&#xae;.

Astrain characterized by (i): sensitivity for ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol antimicrobial activity againstand; and (iii) a sporulation percentage of at least 80 when measured after 2 days of incubation. The invention further relates to a method for selecting such strains. Many of the identified strains according to the invention are of the species. Some of thewere further identified assubsp.whereas others were identified assubsp.. Astrain of the invention may be used as a feed additive to animal feed where it has a probiotic effect.

Antibiotic sensitivestrains having antimicrobial effect againstandand having high sporulation capacity

The present invention relates to mutants of lactic acid bacteria which are resistant towards the antibiotic D-cycloserine and/or functionally equivalent antibiotics and which were found to give an increased texture when grown in milk while maintaining the other growth properties of the parent strain. The present invention, furthermore, relates to compositions comprising such mutants, and to dairy products fermented with the lactic acid bacteria resistant towards D-cycloserine and/or functionally equivalent antibiotics.

Texturizing lactic acid bacteria strains

Astrain characterized by (i): sensitivity for ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol; (ii) antimicrobial activity againstand; and (iii) a sporulation percentage of at least 80 when measured after 2 days of incubation. The invention further relates to a method for selecting such strains. Many of the identified strains according to the invention are of the species. Some of thewere further identified assubsp.whereas others were identified assubsp.. Astrain of the invention may be used as a feed additive to animal feed where it has a probiotic effect.

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Cycloserine resistant mutants of lactic acid bacteria characterized by having improved resistance towards ethanol. The cycloserine resistant mutants of lactic acid bacteria can e.g. be used for malolactic fermentations of wine (i.e. including sparkling wine such as Cava/champagne) having high alcohol levels.

Novel food-grade cloning vectors comprising a nonsense mutation suppressor-encoding gene, which vector, when it is present in a lactic acid bacterial strain, permits such a strain to have an industrially appropriate growth rate and metabolic activity. The cloning vectors are useful when present in lactic acid bacteria used as starter cultures in the preparation of food or feed products, or a dairy flavor.

Food-grade cloning vector and their use in lactic acid bacteria

Lactic acid bacterial suppressor mutants and their use as selective

Recombinant lactic acid bacterium containing an inserted promoter and

Useful mutants of lactic acid bacteria or plasmids capable of replicating in lactic acid bacteria, comprising nonsense mutation suppressor-encoding genes, the use of such suppressor genes for confining a replicon to a specific lactic acid bacterium or to a lactic acid bacterium growing in a particular environment and for controlling the number of lactic acid bacterial cells in a particular environment.

Growing community of inventors

Hoersholm, Denmark

Eric Johansen