Lygos, Inc.

University of California

Eric J Steen

Jeffrey Allen Dietrich

Jeffrey L Fortman

Jay D Keasling

Johan Van Walsem

Leonard Katz

Methods for the preparation of bio-based malonic acid and diester derivatives of malonic acid are provided. For example, a dialkyl malonate may be prepared by the steps of (i) separating calcium malonate crystals from a fermentation broth; (ii) obtaining dissolved malonic acid; (iii) crystallizing the dissolved malonic acid; and (iv) performing esterification to obtain the dialkyl malonate. The disclosed methods produce diester derivatives of malonic acid with fewer impurities, which is useful for many industrial processes. The diester derivatives of malonic acid can be purified from existing sources of malonic acid, or from malonic acid made from a renewable carbon source.

Method for preparation of diester derivatives of malonic acid

A process for the preparation of bio-based malonic acid and crystalline calcium malonate is provided. The calcium malonate is highly pure and provides a source of malonic acid made from a renewable carbon source rather than existing processes which rely on the use of petroleum-based products. The calcium malonate provides an improved source of malonic acid, which is important to many industrial processes.

Process for purification of malonic acid from fermentation broth

Systems and methods for the production of malonate in recombinant host cells.

Recombinant host cells for the production of malonate

The present invention provides for a method of producing one or more fatty acid derived dicarboxylic acids in a genetically modified host cell which does not naturally produce the one or more derived fatty acid derived dicarboxylic acids. The invention provides for the biosynthesis of dicarboxylic acid ranging in length from C3 to C26. The host cell can be further modified to increase fatty acid production or export of the desired fatty acid derived compound, and/or decrease fatty acid storage or metabolism.

Host cells and methods for producing diacid compounds

The present invention provides for a polyketide synthase (PKS) capable of synthesizing an &#x3b1;-olefin, such as 1-hexene or butadiene. The present invention also provides for a host cell comprising the PKS and when cultured produces the &#x3b1;-olefin.

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San Francisco, CA, United States of America

Eric J Steen