New England Biolabs, Inc.

Eric J Cantor

Jennifer Ong

Vladimir Potapov

Ashley Luck

Paul A Koetsier

Barbara W Taron

Heidi Crosby

The present disclosure relates, according to some embodiments, to systems, apparatus, compositions, methods, and workflows that include DNase I variants with desirable properties including, for example, salt tolerance. A DNase I variant, in some embodiments, may have an amino acid sequence that is at least 85% identical, at least 90% identical, at least 95% identical, and/or at least 98% identical to SEQ ID NO:1 and may be identical to SEQ ID NO:1 at one or more positions selected from the group of positions corresponding to L29, A35, D87, Q88, S94, P103, T108, P121, P132, A135, D145, E161, G172, P190, H208, and A224 of SEQ ID NO:1.

DNase I variants, compositions, methods, and kits

Provided herein is a method for isolating high molecular weight (HMW) DNA using beads that are at least 200 &#x3bc;m in diameter that utilizes a device for retaining the beads and where the purified DNA eluant exits the device without shearing the HMW DNA. In some embodiments, the method comprises precipitating the DNA onto the beads, washing the beads in the device, and then eluting the DNA from the beads therein while substantially avoiding shear. Compositions and kits for practicing the method are also provided.

Isolation of high molecular weight DNA using beads

Growing community of inventors

Topsfield, MA, United States of America

Eric J Cantor