Wisconsin Alumni Research Foundation

Eric Huy Dang Nguyen

William Leo Murphy

Michael P Schwartz

Stefan Zorn

Ngoc Nhi Le

Matthew Brian Parlato

William T Daly

David Belair

Ngoc Nhi Thi Le

Hamisha Ardalani

Matthew Zanotelli

David Gregory Belair

Patterned hydrogel arrays and methods of preparing patterned hydrogel arrays are disclosed. Advantageously, the methods used to prepare the patterned hydrogel arrays allow for controlling individual hydrogel spot conditions such as hydrogel spot modulus, hydrogel spot ligand identity and hydrogel spot ligand density, which allows for preparing a wide range of hydrogel spots in a single array format. Patterned hydrogel arrays can also be formed to include hydrogel-free pools surrounded by hydrogel. Additionally, the patterned hydrogel arrays of the present disclosure support the culture of a range of cell types. The patterned hydrogel arrays offer the ability to rapidly screen substrate components for influencing cell attachment, spreading, proliferation, migration, and differentiation.

Method for forming hydrogel arrays using surfaces with differential wettability

Hydrogel Compositions and methods of using hydrogel compositions are disclosed. Advantageously, the hydrogel compositions offer the ability to rapidly screen substrate components for influencing cell attachment, spreading, proliferation, migration, and differentiation. In particularly suitable embodiments, the hydrogel compositions of the present disclosure may be used to promote tubulogenesis of endothelial cells.

Hydrogel compositions for use in promoting tubulogenesis

Growing community of inventors

Madison, WI, United States of America

Eric Huy Dang Nguyen