Thermo Finnigan LLC

Eric Hemenway

Michael W Senko

Vladimir Zabrouskov

Justin Blethrow

Shannon Eliuk Dixon

Tonya Second

Tina A Hemenway

A hybrid mass spectrometer design and architecture, and methods of operating mass spectrometers are disclosed. According to one operating method, an analysis time is determined for each one of a plurality of ion species to be analyzed in an ordered sequence, and an injection time is calculated for at least some of the ion species based on an analysis time of a preceding ion species in the ordered list. The method enables more efficient utilization of analyzer time.

Hybrid mass spectrometer and methods of operating a mass spectrometer

A method of analyzing data from a mass spectrometer for a data dependent acquisition is described. In an embodiment of this method, mass spectral scans are taken of a sample eluted from a liquid chromatography column. An extracted ion chromatogram (XIC) is then created for each m/z data point of the mass spectral scans and the XIC for each m/z data point are correlated to a model function, such as a monotonically increasing function, or the first half of a gaussian function, to obtain a XIC correlation value. A weighting function is then applied to the XIC correlation value to obtain a current weighted intensity. The current weighted intensity for each m/z point is used to reconstruct a weighted mass spectrum, which is then used to make a real-time decision for the data dependent acquisition. In an embodiment, the data dependent acquisition is the performance of tandem mass spectrometry. Embodiments of the invention also describe a sample processing apparatus for the data dependent acquisition and a computer readable medium that provides instructions to the sample processing apparatus to perform the method described above.

Methods for improved data dependent acquisition

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Milpitas, CA, United States of America

Eric Hemenway