Illumina Corporation

Illumina Cambridge Limited

Illumina Singapore Pte. Ltd.

Eric Hans Vermaas

Yong Li

Colin Lloyd Barnes

Jonathan Mark Boutell

Roberto Rigatti

Niall Anthony Gormley

Geoffrey Paul Smith

Ryan Kelley

Tobias William Barr Ost

Vincent Peter Smith

Helmy A Eltoukhy

Tarun Kumar Khurana

Sebastian Bohm

Robert C Kain

Dale Buermann

Frank J Steemers

Allen E Eckhardt

David Bentley

Graham John Worsley

Min-Jui Richard Shen

Arash Jamshidi

Jian Gong

AmirAli Talasaz

Matthew William Hage

Kenneth M Kuhn

Mark T Reed

Mark R Pratt

Harold Philip Swerdlow

Jason Bryant

Rigo Pantoja

John Bridgham

Klaus Maisinger

Anindita Dutta

Andrew Kennedy

Andrew Slatter

Dmitry K Pokholok

Michelle L Alvarez

Fan Zhang

Claire Bevis-Mott

Yin Nah Teo

Gordon Bean

Mahdieh Khosroheidari

Tatjana Singer

George Sakaldasis

Bojan Obradovic

Sarah E Shultzaberger

Isabelle Rasolonjatovo

Lena Christiansen

Andrew Price

Xiangyuan Yang

Angela Kalbande

Johnny Ho

Xavier Lee

Peter Lundberg

Chiara Rodighiero

Subra Sankar

Kevin Benson

Saibal Banerjee

Sergey Etchin

Colin Brown

Matthew Hage

Daniel Ortiz

Jeffrey Brodin

Jonny Ho

Ning Sizto

Sepideh Almasi

Kayla Busby

Matthew Feeley

Patrizia Iavicoli

Megan Chu

Jennifer Zou

Rigoberto Pantoja

Weihua Chang

Jerushah Thomas

The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by modifying or blocking 5' and 3′ ends of pooled indexed polynucleotides from multiple samples, with an optional exonuclease treatment, prior to amplification and sequencing.

Compositions and methods for improving sample identification in indexed nucleic acid libraries

The disclosed embodiments concern index oligonucleotides configured to identify sources of samples of nucleic acids and methods, apparatus, systems and computer program products for identifying and making the index oligonucleotides. In some implementations, the index oligonucleotides include a set of index sequences, a Hamming distance between any two index sequences of the set of index sequences meeting one or more criteria. System, apparatus, and computer program products are also provided for determining a sequence of interest using the index oligonucleotides.

Universal short adapters for indexing of polynucleotide samples

Optimal index sequences for multiplex massively parallel sequencing

The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by exonuclease treatment after protective adapters are ligated to target polynucleotides to degrade unincorporated adapters prior to amplification and sequencing.

The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by modifying or blocking 5' and 3&#x2032; ends of pooled indexed polynucleotides from multiple samples, with an optional exonuclease treatment, prior to amplification and sequencing.

A phase-separation device and method of use is provided for separating immiscible liquids. The phase-separation device has a porous membrane with a filter surface having a non-planar contour that forms a receiving cavity to receive a liquid mixture. The filter surface is configured to impede flow of a polar liquid into the porous membrane and permit flow of a non-polar liquid into the porous membrane.

Method and device for separating immiscible liquids to effectively isolate at least one of the liquids

The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.

Method for sequencing a polynucleotide template

Provided are methods and apparatuses for performing sequencing using droplet manipulation, for example, via electrowetting-based techniques. Also provided are integrated methods and apparatuses for performing sample preparation and sequencing on the same apparatus. In addition, provided are methods of reducing reagent waste and preloaded consumable cartridges comprising reagents for sample preparation and/or sequencing.

Integrated sequencing apparatuses and methods of use

Method that includes providing a phase-separation device having a porous membrane with a filter surface. The filter surface has a non-planar contour that forms a receiving cavity. The method also includes providing a liquid mixture into the receiving cavity of the porous membrane. The liquid mixture includes a polar liquid and a non-polar liquid that are immiscible with respect to each other. The filter surface along the receiving cavity has a surface energy that impedes flow of the polar liquid through the filter surface and permit flow of the non-polar liquid into the porous membrane. The method also includes permitting the non-polar liquid to flow into the porous membrane. The polar liquid forms a droplet within the receiving cavity as the non-polar liquid flows into the porous membrane.

Method for sequencing a polynucelotide template

The present invention comprises systems and devices for sequencing of nucleic acid, such as short DNA sequences from clonally amplified single-molecule arrays.

Systems and devices for sequence by synthesis analysis

Growing community of inventors

Hayward, CA, United States of America

Eric Hans Vermaas