Illumina Corporation

Glaxo Group Limited

Biogen, Incorporated

Illumina Cambridge Limited

Solexa, Inc.

Eric H Kawashima

Pascal Mayer

Laurent Farinelli

Gerardo Turcatti

Celine Adessi

Jean-Jacques Mermod

Gary N Buell

Bernard Allet

Annmarie Surprenant

Jean-Jaques Mermod

Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5' end an oligonucleotide sequence Y and at the 3&#x2032; end an oligonucleotide sequence Z and, in addition, the nucleic acid(s) carry at the 5&#x2032; end a means for attaching the nucleic acid(s) to a solid support; (2) mixing said nucleic acid template(s) with one or more colony primers X, which can hybridize to the oligonucleotide sequence Z and carries at the 5&#x2032; end a means for attaching the colony primers to a solid support, in the presence of a solid support so that the 5&#x2032; ends of both the nucleic acid template and the colony primers bind to the solid support; (3) performing one or more nucleic acid amplification reactions on the bound template(s), so that nucleic acid colonies are generated and optionally, performing at least one step of sequence determination of one or more of the nucleic acid colonies generated. Solid supports, kits and apparatus for use in these methods.

Methods of nucleic acid amplification and sequencing

A nucleic acid molecule can be annealed to an appropriate immobilized primer. The primer can then be extended and the molecule and the primer can be separated from one another. The extended primer can then be annealed to another immobilized primer and the other primer can be extended. Both extended primers can then be separated from one another and can be used to provide further extended primers. The process can be repeated to provide amplified, immobilized nucleic acid molecules. These can be used for many different purposes, including sequencing, screening, diagnosis, in situ nucleic acid synthesis, monitoring gene expression, nucleic acid fingerprinting, etc.

Method of nucleic acid amplification

A nucleic acid molecule can be annealed to an appropriate immobilized primer. The primer can then be extended and the molecule and the primer can be separated from one another. The extended primer can then be annealed to another immobilized primer and the other primer can be extended. Both extended primers can then be separated from one another and can be used to provided further extended primers. The process can be repeated to provide amplified, immobilized nucleic acid molecules. These can be used for many different purposes, including sequencing, screening, diagnosis, in situ nucleic acid synthesis, monitoring gene expression, nucleic acid fingerprinting, etc.

Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5' end an oligonucleotide sequence Y and at the 3&#x2032; end an oligonucleotide sequence Z and, in addition, the nucleic acid(s) carry at the 5&#x2032; end a means for attaching the nucleic acid(s) to a solid support; (2) mixing said nucleic acid template(s) with one or more colony primers X, which can hybridize to the oligonucleotide sequence Z and carries at the 5&#x2032; end a means for attaching the colony primers to a solid support, in the presence of a solid support so that the 5&#x2032; ends of both the nucleic acid template and the colony primers bind to the solid support; (3) performing one or more nucleic acid amplification reactions on the bound template(s), so that nucleic acid colonies are generated and optionally, performing at least one step of sequence determination of one or more of the nucleic acid colonies generated. Solid supports, kits and apparatus for use in these methods.

The present invention relates, in general, to a purinergic receptor and, particular, to a P2X (also designated P2Z) receptor. The invention further relates to a nucleic acid encoding the P2X receptor and to a method of producing P2X combinantly using same. The invention also relates to a method of screening compounds for their ability to inhibit P2X activity and thereby for their usefulness in treating a variety of diseases/disorders, including arthritic and respiratory disorders and neurodegenerative diseases.

Methods of screening modulators of mammalian P2X7 purinergic receptors

The present invention relates, in general, to a purinergic receptor and, in particular, to a P2X.sub.7 (also designated P2Z) receptor. The invention further relates to a nucleic acid encoding the P2X.sub.7 receptor and to a method of producing P2X.sub.7 recombinantly using same. The invention also relates to a method of screening compounds for their ability to inhibit P2X.sub.7 activity and thereby for their usefulness in treating a variety of diseases/disorders, including arthritic and respiratory disorders and neurodegenerative diseases.

Purinergic receptor

A process of producing mammalian tumor necrosis factors (TNF) and TNF-like polypeptides by culturing eukaryotic or prokaryotic hosts transformed with DNA sequences encoding those polypeptides. A process for purifying TNF-like polypeptides using an anion exchanger. The TNFs and TNF-like polypeptides produced by the processes of this invention, and compositions and methods utilizing those TNFs and TNF-like polypeptides, are useful in anticancer, antitumor and antimalarial therapies. They are also useful together with interferon therapy, chemotherapy in anticancer and antitumor therapies, and in combination with actinomycin D in the treatment of tumor-bearing mammals.

Processes for producing tumor necrosis factor

Recombinant DNA molecules comprising DNA sequences derived from bacteriophage T4 that are useful in expressing desired polypeptides in unexpectedly high yields, hosts and expression systems comprising such recombinant DNA molecules and methods for expressing desired polypeptides in high yields by the utilization of such hosts and expression systems.

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Geneva, Switzerland

Eric H Kawashima