Amgen Inc.

Eric F Fisher

Carl Edwards, Iii

Gary Kieft

Graham Beaton

Disclosed are novel proteins, referred to as tumor necrosis factor binding proteins, that modulate the activity of tumor necrosis factor. Also disclosed are processes for obtaining the tumor necrosis binding proteins by recombinant genetic engineering techniques.

Nucleic acids encoding truncated soluble tumor necrosis factor

Truncated soluble tumor necrosis factor type-I and type-II receptors

The present invention provides nuclease resistant 3'-carbon modified oligonucleotides that can be used in the field of nucleic acid therapeutics and diagnostics. The modified oligonucleotides of the present invention have at least one modified internucleotide linkage wherein the divalent oxygen moiety at the 3'-position of the internucleotide linkage is replaced by a tetravalent carbon moiety. The 3'-carbon modified internucleotide linkage is preferably a 3'-methylene or 3'-hydroxymethylene linkage. Also provided are a method and monomeric nucleoside and nucleotide intermediates for making the modified oligonucleotides of the present invention.

Modified oligonucleotides and intermediates useful in nucleic acid

A system for the production of biotin wherein a biotin retention-deficient strain of a cell is transformed with plasmid bearing the biotin gene cluster bio (A, B, F, C and D). The media of cultures of the resulting cells contains enhanced amounts of biotin by comparison with similar constructions in strains capable of biotin retention.

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Boulder, CO, United States of America

Eric F Fisher