University of California

Scienion Gmbh

Scienion Ag

Eric D Chow

Robert Joseph Weber

Zev Jordan Gartner

Joseph L DeRisi

Michael J Wilson

Joshua Cantlon-bruce

David Patterson

Emily D Crawford

Derek Bogdanoff

Christopher Mcginnis

Ariele Lauren Greenfield

Joshua Cantlon-Bruce

Michael Wilson

Disclosed are compositions comprising a first lipid-conjugated oligonucleotide comprising a first lipid moiety, a first hybridization region, and a first primer region; a second lipid-conjugated oligonucleotide comprising a second hybridization region and a second lipid moiety, wherein the second hybridization region is the reverse complement of the first hybridization region; and a third oligonucleotide comprising a second primer region, a barcode region, and a capture sequence, wherein the second primer region is the reverse complement of the first primer region; compositions comprising a lipid-conjugated DNA oligonucleotide comprising a lipid moiety, a barcode region, and a capture sequence; and composition comprising a first lipid-conjugated DNA oligonucleotide comprising a lipid moiety and a first primer region; and a second DNA oligonucleotide comprising a second primer region, a barcode region, and a capture sequence, wherein the second primer region is the reverse complement of the first primer region. Also disclosed are membranes and cells comprising such compositions and uses of such compositions.

Lipid-modified oligonucleotides and methods of using the same

A particle isolation apparatusfor isolating particles from a suspension sample, includes a droplet dispenser devicefor collecting the suspension sample from a carrier substrateand for dispensing droplets onto a target substrate, a mechanical pump devicebeing coupled with the droplet dispenser devicefor loading a dilution liquid into the droplet dispenser deviceand for aspirating a first portion of the suspension sample into the droplet dispenser device, and a syphon pump devicebeing coupled with the droplet dispenser deviceand being arranged for aspirating a second portion of the suspension sample into the droplet dispenser device. Preferably, the droplet dispenser deviceis configured for dispensing single particle droplets on the target substrate. Furthermore, a method of isolating particles from a suspension sample is described.

Apparatus and method for isolating single particles from a particle suspension

A method of sample analysis is provided. In some embodiments, the method comprises: (a) digesting a mixed nucleic acid sample with a plurality of reprogrammed nucleic acid-directed endonucleases that target sequences of interest to produce a digested sample, wherein at least some of the fragments in the digested sample comprise: (i) a sequence of interest and (ii) at least one ligatable end that has been generated by endonuclease cleavage; (b) enriching for fragments that contain the sequence of interest; and (c) analyzing the enriched fragments. Kits for performing the method are also provided.

Method for finding low abundance sequences by hybridization (FLASH)

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San Francisco, CA, United States of America

Eric D Chow