Dow Global Technolgoies LLC

Eric C Shiue

Paresh C Sanghani

Scott A Greenwalt

Christopher C Stowers

Brandon A Rodriguez

Sarah Delaplane

Prakash Bhosale

Genetically modified isopropylmalate isomerase enzyme complexes (e.g., LeuCD′ enzyme complexes), microbial organisms including genetically modified isopropylmalate isomerase enzyme complexes (e.g., LeuCD′), and processes for preparing C7-C11 2-ketoacids with genetically modified isopropylmalate isomerase enzyme complexes (e.g., LeuCD′). The genetically modified isopropylmalate isomerase enzyme complexes (e.g., LeuCD′ enzyme complexes), microbial organisms, and processes for preparing C7-C11 2-ketoacids can be used to produce C6-C10 aldehydes, alkanes, alcohols, and carboxylic acids, both in vivo and in vitro.

Genetically modified isopropylmalate isomerase enzyme complexes and processes to prepare elongated 2-ketoacids and C5-C10 compounds therewith

Genetically modified LeuCD' enzyme complexes, processes for preparing a C-C2-ketoacid utilizing genetically modified LeuCD&#x2032; enzyme complexes, and microbial organisms including modified LeuCD enzyme complexes are described. The instantly-disclosed genetically modified LeuCD&#x2032; enzyme complexes, processes for preparing a C-C2-ketoacid, and microbial organisms including modified LeuCD&#x2032; enzyme complexes can be particularly useful for producing C-Caldehydes, alkanes, alcohols, and carboxylic acids, both in vivo and in vitro.

Process to prepare elongated 2-ketoacids and C-5-C10 compounds therefrom via genetic modifications to microbial metabolic pathways

Genetically modified isopropylmalate isomerase enzyme complexes (e.g., LeuCD' enzyme complexes), microbial organisms including genetically modified isopropylmalate isomerase enzyme complexes (e.g., LeuCD&#x2032;), and processes for preparing C-C2-ketoacids with genetically modified isopropylmalate isomerase enzyme complexes (e.g., LeuCD&#x2032;). The genetically modified isopropylmalate isomerase enzyme complexes (e.g., LeuCD&#x2032; enzyme complexes), microbial organisms, and processes for preparing C-C2-ketoacids can be used to produce C-Caldehydes, alkanes, alcohols, and carboxylic acids, both in vivo and in vitro.

Genetically modified isopropylmalate isomerase enzyme complexes and processes to prepare elongated 2-ketoacids and C-Ccompounds therewith

Modification of the amino acid sequence of a phenylpyruvate decarboxylase fromproduces a novel group of phenylpyruvate decarboxylases with improved specificity to certain substrates, including in particular C7-C11 2-ketoacids such as, for example, 2-ketononanoate and 2-keto-octanoate. This specificity enables effective use of the phenylpyruvate decarboxylase in, for example, an in vivo process wherein 2-ketobutyrate or 2-ketoisovalerate are converted to C7-C11 2-ketoacids, and the novel phenylpyruvate decarboxylase converts the C7-C11 2-ketoacid to a C6-C10 aldehyde having one less carbon than the 2-ketoacid. Ultimately, through contact with additional enzymes, such C6-C10 aldehydes may be converted to, for example, C6-C10 alcohols, C6-C10 carboxylic acids, C6-C10 alkanes, and other derivatives. Use of the novel genetically modified phenylpyruvate decarboxylases may represent a lower cost alternative to non-biobased approaches.

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Evanston, IL, United States of America

Eric C Shiue