Syngenta Participations Ag

Pieris Ag

Pieris Pharmaceuticals Gmbh

Eric Boudreau

Arne Skerra

Andreas Hohlbaum

Kristian Hobolt Jensen

Martin Huelsmeyer

Steffen Schlehuber

Richard Jones

Ian Kimber

Rebecca Dearman

Weining Gu

Heng Zhong

Peter Bernard Heifetz

Erik M Dunder

Yin-Fu Chang

Gerson Graser

Anic Deframond

Sabrina Rouse

Richard C Jones

Erik Martin Dunder

Sabrina Noel Rouse

The present invention relates to novel muteins derived from human tear lipocalin. The invention also refers to a corresponding nucleic acid molecule encoding such a mutein and to a method for its generation. The invention further refers to a method for producing such a mutein. Finally, the invention is directed to a pharmaceutical composition comprising such a lipocalin mutein as well as to various uses of the mutein.

Muteins of tear lipocalin and methods for obtaining the same

The present invention relates to isolated nucleic acid molecule comprising the untranslated region of SEQ ID NO: 1, 2 or 3. The invention also relates to chimeric genes comprising SEQ ID NO: 2 operatively linked to the coding sequence of a gene of interest and to plant transformation vectors comprising the chimeric genes of the invention. The invention further relates to transgenic plants, plant cells, plant seeds, plant tissues, or plant plastids comprising the chimeric gene.

Promoters functional in plant plastids

The present invention relates to isolated nucleic acid molecule comprising the untranslated region of SEQ ID NO:2, which is functional as a promoter in plant plastids. The invention also relates to chimeric genes comprising SEQ ID NO:2 operatively linked to the coding sequence of a gene of interest and to plant transformation vectors comprising the chimeric genes of the invention. The invention further relates to transgenic plants, plant cells, plant seeds, plant tissues, or plant plastids comprising the chimeric genes.

The present invention relates to the field of plastid transformation. The invention provides transformation vectors and methods to obtain transplastomic plants or algae having a transformed plastid comprising the steps of introducing into plastids a recombinant nucleic acid molecule or vector, and two phases of selection with first selection phase using a non-lethal compound and a second selection phase using a lethal compound. Alternatively, the dual selection method is conducted simultaneously using a lower concentration of the lethal compound.

Plant cell plastid transformation method using dual selection and producing transplastomic plants without antibiotic resistance genes

The present invention relates to isolated nucleic acid molecule comprising a nucleic acid promoter or untranslated region comprising the nucleic acid sequence of SEQ ID NO: 1, 2 or 3. The invention also relates to isolated nucleic acid promoter or untranslated region comprising the nucleic acid sequence of SEQ ID NO: 1, 2 or 3. The invention further relates to chimeric genes comprising the isolated nucleic acid promoter or untranslated region of SEQ ID NO:1, 2 or 3 operatively linked to the coding sequence of a gene of interest. The invention also relates to plant transformation vectors comprising the chimeric genes of the invention. The invention further relates to transgenic plants, plant cells, plant seeds, plant tissues, or plant plastids, comprising the chimeric genes of this invention.

Multiple shoot structures are induced from plant tissues (e.g., shoot apices or axillary buds on an artificial medium) to produce multiple shoot cultures. These multi-shoot cultures are then transformed by known transformation methods. Plants are subsequently regenerated from the transformed cells. Crops that may be efficiently transformed by this method include plants normally recalcitrant to transformation such as sugar beet, sunflower, soybean, cotton, tobacco, tomato, peanuts, melons, watermelon, squash,and pepper.

Methods for stable transformation of plants

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Research Triangle Park, NC, United States of America

Eric Boudreau