Ajinomoto Co., Ltd.

Eri Tabuchi

Uno Tagami

Kenichi Mori

Takayuki Ito

Shunichi Suzuki

Masakazu Sugiyama

Hiroyuki Nozaki

Tatsuki Kashiwagi

Hideyuki Suzuki

Yasuaki Takakura

Hidemi Fujii

Ayako Sasahara

Hiroomi Ogino

Koki Ishikawa

A mutant glutamate-cysteine ligase (GSHA) suitable for generating &#x3b3;-Glu-Val, and a method for producing &#x3b3;-Glu-Val-Gly using the same are provided. &#x3b3;-Glu-Val is produced by using a mutant GSHA having a specific mutation with Glu and Val as raw materials, and &#x3b3;-Glu-Val-Gly is further produced by using &#x3b3;-Glu-Val and Gly as raw materials. &#x3b3;-Glu-Val-Gly is produced by using a mutant GSHA having a specific mutation with Glu, Val, and Gly as raw materials.

Mutant glutamate-cysteine ligase and method for manufacturing gamma glutamyl-valyl-glycine

The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.

Method for producing monatin using an L-amino acid aminotransferase

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Kawasaki, Japan

Eri Tabuchi