Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Georg-august-universitat Gottingen Stiftung Des Offentlichen Rechts

Erdmann Rapp

Udo Reichl

Christian Bohne

Jana Schwarzer

Stefan W Hell

Vladimir Belov

Henning Urlaub

Gyuzel Mitronova

Ales Charvat

Bernd Abel

Matthias Bischoff

Dirk Meineke

Elizaveta Savicheva

Laura Thomas

Kirill Kolmakov

Marvin J Boehm

René Hennig

Jana Bohne

Sulfonated 2(7)-aminoacridone and 1-aminopyrene dyes and their use as fluorescent tags, in particular for carbohydrate analysis The invention relates to fluorescent dyes with multiple negatively charged groups in their ionized form which are aminoacridone sulfonamides or 1-aminopyrenes having of one of the following general formulae A-D: Formula (A), Formula (B), Formula (C), Formula (D), wherein the ionizable groups X are typically selected from the following: SH, COOH, SOH, OSOH, OP(O)(OH), OP(O)(OH)R, P(O)(OH), P(O)(OH)R, where R═C-Calkyl or substituted C-Calkyl. The invention further relates to the use of these dyes as fluorescent tags, in particular for reducing sugars and glycans.

Sulfonated 2(7)-aminoacridone and 1-aminopyrene dyes and their use as fluorescent tags, in particular for carbohydrate analysis

The present invention relates to methods for the identification of compounds in carbohydrate mixture compositions as well as the determination of carbohydrate mixture composition patterns, based on e.g. orthogonal cross determining migration time (indices) using capillary gel electrophoresis-laser induced fluorescence and identifying said carbohydrate components based on comparing said migration time (indices) with standard migration time (indices) from a database which data are preferably also orthogonal cross determined. In a further aspect, the present invention relates to a method for carbohydrate mixture composition pattern profiling, like glycosylation pattern profiling using capillary gel electrophoresis-laser induced fluorescence (CGE-LIF). In another aspect, the present invention refers to a system for an automated determination and/or identification of carbohydrates and/or carbohydrate mixture composition patterns (e.g.: glycosylation patterns). Finally, the present invention relates to a database containing e.g. orthogonal cross normalized migration times and/or migration time indices of carbohydrates.

Method for automated high throughput identification of carbohydrates and carbohydrate mixture composition patterns as well as systems therefore

The invention relates to a method and an apparatus for providing a sample for a subsequent analysis of the sample, particularly for analyzing biomolecules, comprising the following steps: generating a free micro liquid jet in an environment having a predetermined pressure, wherein the micro liquid jet contains a carrier liquid and the sample to be analyzed, and dispersing the micro liquid jet into droplets containing the sample, wherein the environment surrounding the micro liquid jet is a gaseous environment in which the pressure is above vacuum conditions.

Method and apparatus for providing a sample for a subsequent analysis

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Magdeburg, Germany

Erdmann Rapp