Centre National De La Recherche Scientifique

Biomerieux, Inc.

Institut National De La Recherche Agronomique

Université Joseph Fourier

Universite Joseph Fourier Domaine

Institut National Des Sciences Appliquees De Toulouse

Dendris

Emmanuelle Trevisiol

Ali Laayoun

Jean Lhomme

Bernard Meunier

Jean-Pierre Majoral

Anne-Marie Caminade

Mitsuharu Kotera

Geneviève Pratviel

Julien Leclaire

Cecile Bourget

Jean Francois

Lionel Menou

Christophe Vieu

Christelle Tora

Eloy Bernal Mendez

Isabelle Sothier

Eric DeFrancq

Antoine Hoang

Eric Defranc

Fran&ccedil;oise Guillou-Bonnici

Jean-Marie Francois

Childerick Severac

Catherine Tardin

Thomas Plenat

Julie Foncy

Christophe Thibault

Laurence Salome

Cécile Bourget

The present invention relates to solid supports functionalized with phosphorus-containing dendrimers, to a process for preparing them, to their use for preparing biochips and to the uses of these biochips, in particular for immobilizing molecules of interest, especially biological molecules of interest such as nucleic acids, polypeptides, lipids and proteins.

Solid supports functionalized with phosphorus-containing dendrimers, process for preparing them and uses thereof

Solid supports functionalised with phosphorus dendrimers, method for preparing same and uses thereof

The present invention relates to a temperature-stable labeling reagent of formula: in which The present invention also describes a method for synthesizing said markers as well as applications for labeling biological molecules, in particular nucleic acids, with a labeling reagent carrying the diazomethyl functional group. The invention finds a preferred application in the field of diagnosis.

Labeling reagents, methods for synthesizing such reagents and methods for detecting biological molecules

A single- or double-stranded deoxyribonucleic acid (DNA) is fragmented and labeled by chemically fragmenting the DNA by creating at least one abasic site on the DNA, and attaching a marker to at least one of the fragments by means of a labeling reagent, the reagent covalently and predominantly coupling to at least one phosphate of the fragment. This method can be applied in the field of diagnosis.

Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling

The invention relates to a functionalized compound of general formula (I): A functionalized polynucleotide comprising at least one said compound and a labeled functionalized polynucleotide comprising at least one functionalized compound corresponding to the formula (I'). A method for detecting a target nucleic acid using a said compound is described.

Functionalized polynucleotide compound, optionally marked and method for detecting a target nucleic acid

A target nucleic acid sequence amplification method wherein at least the target nucleic acid sequence, at least one oligonucleotide primer specific for the target sequence, one or more enzymatic activities and nucleotides are provided, and the target sequence is amplified under conditions suitable in particular for said enzymatic or activity or activities, is disclosed. According to the method, at least one of the nucleotides is a prefunctionalized nucleotide that differs from the other nucleotides at least in that it includes at least one unprotected reactive covalence function in at least one predetermined site of the base of said nucleotide, in order to give a prefunctionalized amplificati product including at least one such prefunctionalized nucleotide. Nucleotides for carrying out the method are also disclosed.

Prefunctionalized nucleotide and process for amplifying a sequence using a prefunctionalized nucleotide

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Cornebarrieu, France

Emmanuelle Trevisiol