Viacyte, Inc.

Cythera, Inc.

Harvard College

Dana-farber-cancer Institute Inc.

Emory University

Brigham and Women's Hopsital, Inc.

Emmanuel Edward Baetge

Kevin Allen D'Amour

Alan D Agulnick

Evert Kroon

Laura A Martinson

Susan Eliazer

Chad Green

Kevin Allen D'amour

Anne Bang

Olivia Kelly

Melissa K Carpenter

Xiaojie Yu

Thomas C Schulz

Allan J Robins

Val Anthony Bellora

Richard Alexander Grant

Susan Eliazar

Eugene P Brandon

Kevin A D'amour

Kevin D' Amour

Olivia G Kelly

Mark Moorman

Disclosed herein are cell cultures and enriched cell populations of endocrine precursor cells, immature pancreatic hormone-expressing cells and mature pancreatic hormone-expressing cells. Also disclosed herein are methods of producing such cell cultures and cell populations.

Methods of using PDX1-positive pancreatic endoderm cells and endocrine precursor cells

Disclosed herein are methods of producing pancreatic hormone-expressing cells by first differentiating pluripotent cells in cell culture so as to produce endodermal cells, the endodermal cells being competent to further differentiate into hormone-expressing cells capable of secreting at least one pancreatic hormone in response to a physiological signal, and then, transplanting the cultured endodermal cells into an organism, such as an organism in need of an endocrine cell therapy.

Methods of producing pancreatic hormones

Disclosed herein are cell cultures comprising PDX1-positive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified PDX1-positive endoderm cells as well as methods for enriching, isolating and purifying PDX1-positive endoderm cells from other cell types. Methods of identifying differentiation factors capable of promoting the differentiation of endoderm cells, such as PDX1-positive foregut endoderm cells and PDX1-negative definitive endoderm cells, are also disclosed.

PDX1 positive foregut endoderm cells and methods of production

This disclosure relates to compositions comprising human preprimitive streak cells and/or human mesendoderm cells as well as methods for their production. Additionally, disclosed herein are methods of identifying factors useful in the further differentiation of preprimitive streak and mesendoderm cell types.

Methods of making human primitive ectoderm cells

The present invention relates to a cryopreserved in vitro cell culture comprising human pancreatic progenitor cells that co-express pancreatic-duodenal homeobox factor-1 (PDX1) and NK6 homeobox 1 (NKX6.1) and are chromogranin negative. The present invention also relates to a method for cryopreserving an in vitro population of human pancreatic progenitor cells that co-express PDX1 and NKX6.1 and are chromogranin negative.

Cryopreserved in vitro cell culture of human pancreatic progenitor cells

Disclosed herein are cell cultures comprising dorsal and/or ventral PDX1-positive foregut endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified dorsal and/or ventral PDX1-positive foregut endoderm cells as well as methods for enriching, isolating and purifying dorsal and/or ventral PDX1-positive foregut endoderm cells from other cell types. Methods of identifying differentiation factors capable of promoting the differentiation of dorsal and/or ventral PDX1-positive foregut endoderm cells, are also disclosed.

Methods of producing human foregut endoderm cells expressing PDX1 from human definitive endoderm

Methods of making and using PDX1-positive pancreatic endoderm cells

Methods of lowering blood glucose levels in a mammal

Anterior endoderm cells and methods of production

Disclosed herein are cell cultures comprising definitive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified definitive endoderm cells as well as methods for enriching, isolating and purifying definitive endoderm cells from other cell types.

Definitive endoderm

Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production

The present invention relates to a perforated semi-permeable device comprising, human pancreatic endocrine cells or human PDX1-positive pancreatic endoderm cells contained within a semi-permeable membrane comprising a synthetic material, wherein the synthetic material is polysulfone (PSF), nano-fiber mats, polyimide, tetrafluoroethylene/polytetrafluoroethylene (PTFE), expanded polytetrafluoroethylene (ePTFE), polyacrylonitrile, polyethersulfone, acrylic resin, cellulose acetate, cellulose nitrate, polyamide, or hydroxylpropyl methyl cellulose (HPMC), a cell encapsulation chamber, and at least one seal that is within the cell encapsulation chamber.

Encapsulation of pancreatic cells derived from human pluripotent stem cells

Growing community of inventors

Encinitas, CA, United States of America

Emmanuel Edward Baetge