Ecole Polytechnique

Centre National De La Recherche Scientifique

Emmanuel Beaurepaire

Marc Hanna

Delphine Debarre

Jean-Louis Martin

Pierre Mahou

Thibault Vieille

Willy Supatto

Khmaies Guesmi

Philippe Rigaud

Frédéric Druon

Lamiae Abdeladim

Maire-Claire Schanne-Klein

Nelly Vuillemin

The invention relates to a device comprising:

Multiphotonic microscopy method and device

A method and system for calibrating an optical device having a spatial optical modulator () and including: an optical lens () of a large numerical aperture, capable of receiving an incident beam and/or collecting an optical beam reflected by a sample; a camera () disposed in a plane optically conjugate with the focal plane of the lens (); and a spatial optical modulator () disposed on the optical beam upstream of the camera (). The method includes the following steps consisting in: acquiring three-dimensional images of the PSF in terms of intensity in a plane conjugate with the focal plane of the lens (), reconstructing the profile in terms of amplitude and/or phase of the beam using a phase diversity algorithm, and determining the respective influence functions of a plurality of elements P, . . . , Pof the spatial optical modulator () on the profile in terms of the amplitude A and/or phase &#x3c6; of the beam.

Method and system for calibrating a spatial optical modulator in an optical microscope

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Palaiseau, France

Emmanuel Beaurepaire