Life Technologies Corporation

Other

Emily Zeringer

Richard Conrad

Alexander Vlassov

Mu Li

Kelly Li

Marie Gonzalez

Ioanna Pagani

Boli Huang

Richard C Conrad

Nucleic acid reagents and corresponding methods of using the same for monitoring and evaluating nucleic acid extraction and amplification reactions.

Nucleic acid extraction and amplification controls and methods of use thereof

Methods of directly isolating nucleic acid from an embedded biological sample are provided. An emulsified digest is generated in the presence of a thermostable protease, and an additive selected from an alkylene glycol, a poly(alkylene glycol), or a block copolymer having an average Mof 76 to 2900, or a salt or derivative or combination thereof. Nucleic acid is isolated directly from the emulsified digest. The methods eliminate the use of organic solvents such as xylene in a deparaffinization step prior to isolating nucleic acids from paraffin-embedded samples, for example.

Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples

Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

Methods and compositions for exosome isolation

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Austin, TX, United States of America

Emily Zeringer