Johns Hopkins University

Emily E Smith

Charles Young

Alex J Proescher

A kit for separating and concentrating nucleic acid and protein targets includes labeled reagents which affect simultaneous co-purification and concentration of a nucleic acid and a protein, a gel isotachophoresis separation unit to which a sample comprising the nucleic acid and the protein is added, a detection unit for the detection of the presence of the nucleic acid and the protein, and instructions for use. The gel electrophoresis includes a gel box having a negative electrode side and a positive electrode side, the negative electrode side being filled with a first buffer comprising 2-Hydroxy-N-(tris(hydroxymethyl)methyl)-3-aminopropanesulfonic acid buffer and the positive electrode side being filled with a second buffer being different than the first buffer. The gel isotachophoresis separation is configured to subject the sample to isotachophoresis using a voltage.

Kit for co-purification and concentration of DNA and proteins using isotachophoresis

A method of simultaneously co-purifying and concentrating nucleic acid and protein targets is described. The method includes automation of the entire sample preparation process, performed by having an analyst add a sample into a device that performs all of the steps necessary to prepare a sample for analysis. The method provides for samples that are not split during the sample preparation process and where common purification methods can be used for purifying multiple analytes.

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Baltimore, MD, United States of America

Emily E Smith