Richter Gedeon Vegyeszeti Gyar Rt

Emilia Simonovits

Imre Moravcsik

Gabor Ambrus

Istvan M Szabo

Tibor Lang

Karoly Albrecht

Eva Ilkoy

Gabor Szepesi

Janos Kiss

Antonia Jekkel Nee Bokany

Laszlo Szemler

Kalman Konczol

Gabor Hantos

Andras Selmeczi

Bela Johan

Laszlo Kuti

Erzsebet Kovacs Nee Komoroczy

Tamas Szontagh

Denes Szekely

Judit Bekes Nee Erdos

Gyorgy Hangay

Andras Gulyas

Judit Vincze Nee Kutrovics

Zsuzsanna Lengyel Nee Szemenyei

Peter Keseru

Eva Csajagi

Pal Putz

Zsuzesanna Vida

Katalin Ranky Nee Szita

Andras Kelen

Aniko Hargitai Nee Franyo

The invention relates to a novel cosmetic and paramedicinal active ingredient composition, particularly with an antiinflammatory and skin-calming effect, which comprises

Cosmetic and paramedicinal compositions containing plant extracts

The invention relates to a process for the production of 9.alpha.-hydroxy-4-androstene-3,17-dione from natural sterols of plant or animal origin or from mixtures thereof by the submerged aerobic fermentation of an enzyme-deficient, sterol-de-grading microorganism in a nutrient medium containing utilizable carbon and nitrogen sources as well as mineral salts, and by isolating the product formed, which comprises transforming the sterol or sterol mixture with the culture of a 1,2-steroid-dehydrogenase deficient strain of new Mycobacterium roseum species which is able to degrade the side-chain of natural sterols, preferably with Mycobacterium roseum sp. nov. 1108/1 deposited at the National Collection of Agricultural and Industrial Microorganisms, Budapest, Hungary under the number NCAIM B (P) 000339, and by isolating and purifying the 9.alpha.-hydroxy-4-androstene-3,17-dione obtained.

Microbiological process for preparing

The invention relates to a process for the production of a fermentation broth with increased vitamin B.sub.12 content by a fermentation process carried out with a methane-producing mixed bacterium population under anaerobic septic conditions in the presence of known nutrient components and precursors. According to the invention, methanol, preferably in an amount of 0.5 v/v %, is added for some days, preferably for 3 days to the fermentation broth containing vitamin B.sub.12 used as the starting substance. Thereafter a minor part, preferably 10% of the fermentation broth, is removed and an equal volume of a nutrient broth containing the usual components in tenfold concentrations is added periodically in every 5th to 12th day, then the removal of fermentation broth is interrupted for 0 to 2 days, preferably for 1 day, and only 0.4 to 1.5 v/v% of methanol are added to the fermentation broth, and subsequently a minor part, preferably 10% of the fermentation broth, is removed daily and an equal volume of a nutrient broth containing the usual components in the usual concentrations is added together with 0.4 to 1.5 v/v % of methanol, depending on the biogas production of the fermentation broth. This series of operations is repeated periodically during the complete fermentation procedure taking into consideration the pH of the fermentation broth.

Process for the production of fermentation broth with increased vitamin

Growing community of inventors

Budapest, Hungary

Emilia Simonovits