Stellenbosch University

Lallemand Hungary Liquidity Management LLC

Universiteit Stellenbosch

Emile Van Zyl

Vineet Rajgarhia

Merja Penttilä

Riaan Den Haan

Danie Lagrange

John E McBride

Charles F Rice

Elena E Brevnova

Matti Siika-Aho

Erin Wiswall

Mark Mellon

Marja Ilmen

Anne K Warner

Haowen Xu

Kristen M Deleault

Alan Benjamin Gilbert

David Anthony Hogsett

Indraneel Shikhare

Jaana Uusitalo

Shaunita Hellouise Rose

Deidre Willes

Elena Brevnova

Jim Flatt

Naomi Thorngren

Emily A Stonehouse

Jeff Villari

Alan Froehlich

Dan Murphy

Heidi Hanson Hau

Jeffrey D Keating

Chhayal Ghandi

Shaunita Rose

Merja Penttila

The present invention provides for heterologous expression of polypeptides encoded by wild-type and condon-optimized variants of cbh1 and/or cbh2 from the fungal organisms(),(),(), and() in host cells, such as the yeast. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

Nucleic acids encoding fungal cellobiohydrolases for expression in yeast

The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-optimized cbh2 genes from the organisms, andsp. in host cells, such as the yeast. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

Heterologous expression of fungal cellobiohydrolase 2 genes in yeast

The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-optimized variants of cbh1 and/or cbh2 from the fungal organisms(),(),(), and() in host cells, such as the yeast. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction.

Yeast cells expressing an exogenous cellulosome and methods of using the same

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Stellenbosch, South Africa

Emile Van Zyl