Roche Nimblegen, Inc.

Ventana Medical Systems, Inc.

Cellular Dynamics International, Inc.

Fujifilm Cellular Dynamics, Inc.

Emile Nuwaysir

Roland D Green

Deepika Rajesh

Amanda Mack

Todd Richmond

Jason Norton

Elizabeth Rondon Dominguez

Kate Nuwaysir

Randy Learish

Matthew Brown

Thomas J Albert

Steve Smith

Nicholas Seay

Chris Kendrick-Parker

Methods and compositions relating to the production of induced pluripotent stem cells (iPS cells) are disclosed. For example, induced pluripotent stem cells may be generated from CD34hematopoietic cells, such as human CD34blood progenitor cells, or T cells. Various iPS cell lines are also provided. In certain embodiments, the invention provides novel induced pluripotent stem cells with a genome comprising genetic rearrangement of T cell receptors.

Reprogramming T cells and hematopoietic cells

The present invention provides methods for optimizing oligonucleotide hybridization probes for use in basic and clinical research. Specifically, the invention involves hybridizing serially diluted genomic sample to the oligonucleotide probes on the array, such that a signal intensity is produced for each of the probes; computationally identifying optimized probes which exhibit signal intensities that correspond to the serial dilutions of genomic sample and are reproducibly strong relative to non-optimized probes.

Optimized probe selection method

The present invention is an improved method of resequencing DNA using microarrays to rapidly map and identify SNPs, deletions and amplification events present in the genome of an organism. The method is performed by hybridizing a reference and a test genome to two separate arrays with each array exhibiting a specific intensity pattern. The intensity differences between the reference and the test genome arrays are used to produce a mutation map. The mapped differences are resequenced on a set of resequencing arrays to identify specific genetic mutations.

Comparative genomic resequencing

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Madison, WI, United States of America

Emile Nuwaysir