Dekalb Genetics Corporation

Emil M Orozco

David A Walters

Paul C Anderson

Albert P Kausch

William J Gordon-Kamm

Catherine J Mackey

Peter M Orr

Michael A Stephens

Christopher E Flick

Donald S Walters

Paul S Chomet

Michael T Mann

Peggy G Lemaux

Thomas R Adams

T Michael Spencer

James V O'Brien

Richard J Daines

Nancy G Willetts

Lucille B Laccetti

William G Start

Sheryl A Chambers

Thomas B Rice

Mary L Mangano

Clayton S Vetsch

Kimberly Glassman

Susan J Zachwieja

This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs thebar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof

This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

Growing community of inventors

Groton, CT, United States of America

Emil M Orozco