Sumitomo Chemical Company, Limited

Emiko Kawano

Satoshi Mitsuda

Hideo Hirohara

Kazuo Kumagai

Hidefumi Yamamoto

Misao Miki

Hisao Yamaguchi

Mamoru Hattori

There is disclosed a process for the production of biotin, in which a culture of a microorganism of the genus Sphingomonas, having an ability to produce biotin, is prepared in a medium, and biotin produced and accumulated in the medium is collected. Also disclosed are microorganisms of the genus Sphingomonas, having an ability to produce biotin, which are useful for this production process of the present invention.

Process for production of biotin using bacteria belonging to the genus

Effective disinfection and washing of immobilized lactase from Aspergillus oryzae is carried out after a period of hydrolyzing lactose by treatment of the immobilized lactase with aqueous solutions of pH's 2 to 4 and 6 to 8.7, one or both of which contain a disinfectant. The solution having a pH of 6 to 8.7 may be a phosphate buffered solution. The lactase may be immobilized on a high polymer compound by covalent linkage. The immobilized lactase has outstanding resistance to chemicals and wide pH stability so that decrease of lactase activity is minimized.

Method for disinfecting and washing of immobilized lactase

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Osaka, Japan

Emiko Kawano