Advanced Cell Diagnostics, Inc.

Emerald Doolittle

Yuling Luo

Steve (Aihua) Chen

Xingyong Wu

Nan Su

Xiaoming Wang

Xiao-Jun Ma

Helen Jarnagin

Bingqing Zhang

Xiao Yan Pimentel

Steve Chen

The present invention relates to detection of nucleic acids and provides a composition comprising a Signal Generating Complex, wherein the composition comprises: (A) a pair of target probes (TPs), wherein a first TP of the pair of TPs comprises a nucleic acid sequence comprising two segments; (B) a pair of base PPAs comprising the first and second base PPAs, wherein the first base PPA comprises a nucleic acid sequence comprising three segments; (C) a set of extension PPAs comprising the first and second extension PPAs, wherein the first extension PPA comprises a nucleic acid sequence comprising two segments; (D) a plurality of pre-amplifiers (PAs), wherein the PAs comprise a nucleic acid sequence comprising three segments; (E) a plurality of amplifiers (AMPs), wherein the AMPs comprise a nucleic acid sequence comprising two segments; and (F) a plurality of label probes (LPs), wherein the LPs comprise a nucleic acid sequence comprising two segments.

Methods to further enhance signal amplification for the in situ detection of nucleic acids

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Newark, CA, United States of America

Emerald Doolittle