Cytonome/st, LLC

Emanuel Tito Mendes Machado

Johnathan Charles Sharpe

Donald Francis Perrault, Jr

Blair D Morad

Rudolf Hulspas

Nemanya Sedoglavich

Johnathan Charles

Stuart Buddy Ehrlich

Donald Frances Perrault

Ruud Hulspas

The present disclosure relates to optical crosstalk reduction in particle processing (e.g., cytometry including flow cytometry using microfluidic based sorters, drop formation based sorters, and/or cell purification) systems and methods in order to improve performance. More particularly, the present disclosure relates to assemblies, systems and methods for minimizing optical crosstalk during the analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting, monitoring and/or enriching) of particles (e.g., cells, microscopic particles, etc.). The exemplary systems and methods for crosstalk reduction in particle processing systems (e.g., cell purification systems) may be particularly useful in the area of cellular medicine or the like. The systems and methods may be modular and used singly or in combination to optimize cell purification based on the crosstalk environment and specific requirements of the operator and/or system.

Assemblies and methods for reducing optical crosstalk in particle processing systems

Systems, methods and non-transitory storage medium are disclosed herein for adjusting an output of a particle inspection system representative of a particle characteristic for a particle flowing in a flow-path of a particle processing system. More particularly, the output may be processed and a calibrated output of the particle characteristic generated. In other embodiments, one or more calibration particles are used. Thus, an output of a particle inspection system representative of a particle characteristic for one or more calibration particles flowing in a flow-path of a particle processing system may be compared relative to a standard and an action may be taken based on a result of the comparing the output to the standard.

Particle processing systems and methods for normalization/calibration of same

A microfluidic multiple channel particle analysis system which allows particles from a plurality of particle sources to be independently simultaneously entrained in a corresponding plurality of fluid streams for analysis and sorting into particle subpopulations based upon one or more particle characteristics.

Multiple flow channel particle analysis system

A microfluidic multiple channel particle analysis system () which allows particles () from a plurality of particle sources () to be independently simultaneously entrained in a corresponding plurality of fluid streams () for analysis and sorting into particle subpopulations () based upon one or more particle characteristics ().

A particle analyzing and/or sorting apparatus and the associated methods. One aspect of the described embodiments relates to an analyzer, or a sorter, having acquisition and sort electronics in the form of a field programmable gate array for processing detected signals. Another aspect relates to a droplet based approach of analyzing and sorting particles and may further include a dynamic element, such a dynamic drop delay. In still another broad aspect, an apparatus and method for dynamically varying other sorting parameters.

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Merrimack, NH, United States of America

Emanuel Tito Mendes Machado