Hoffmann-la Roche Inc.

Emanuel Grunberg

Manfred Weigele

Willy Leimgruber

Roy Cleeland, Jr

Max Hoffer

Magdalena U Gomez

Compounds of the formula ##STR1## wherein R.sub.1 is lower alkyl or phenyl lower alkyl; R.sub.2 is phenyl or substituted phenyl; and R.sub.3 is substituted or unsubstituted phenyl, naphthyl or indolyl; are fluorogenic reagents which react with primary amino groups to form fluorophors. The above compounds are useful for fluorescent labeling of materials of biological importance.

Fluorogenic materials and labeling techniques

Compounds of the formula ##STR1## wherein R.sub.1 is lower alkyl or phenyl lower alkyl; R.sub.2 is phenyl or substituted phenyl; and R.sub.3 is substituted or unsubstituted phenyl, naphthyl or indolyl; Are fluorogenic reagents which react with primary amino groups to form fluorophors. The above compounds are useful for fluorescent labeling of materials of biological importance.

1-Dimethylamino-2,4-diphenyl-1-butene-3,4-dione

Compounds of the formula ##STR1## wherein R.sub.1 is lower alkyl or phenyl lower alkyl: R.sub.2 is phenyl or substituted phenyl: and R.sub.3 is substituted or unsubstituted phenyl, naphthyl or indolyl; Are fluorogenic reagents which react with primary amino groups to form fluorophors. The above compounds are useful for fluorescent labeling of materials of biological importance.

Certain 2-hydroxy-2,4-diaryl-3-furanones

Compounds of the formula ##STR1## wherein R.sub.1 is lower alkyl or phenyl lower alkyl: R.sub.2 is phenyl or substituted phenyl; and R.sub.3 is substituted or unsubstituted phenyl, naphthyl or indolyl; Are fluorogenic reagents which react with primary amino groups to form fluorophors. The above compounds are useful for fluorescent labeling of materials of biological importance.

Antibacterial compositions containing 5-sulfanilamido-3,4-dimethylisoxazole and 2,4-diamino-5-(3,4,5-trimethoxybenzyl)-pyrimidine are described.

Composition containing 5-sulfanilamido-3,4-dimethylisoxazole and a

2,4-Diamino-5-(3-alkoxy-4,5-methylenedioxybenzyl)-pyrimidines, such as, for example, 2,4-diamino-5-(3-methoxy-4,5-methylenedioxybenzyl)pyrimidine, prepared from the corresponding benzaldehydes, are described. The end products are useful as potentiators of the antibacterial activity of sulfonamides, as well as antibiotics.

Antibacterial compositions

Fluorogenic 2-oxy-3(2H)-furanone materials

2,4-Diamino-5-(3-alkoxy-4,5-methylenedioxybenzyl)pyrimidines, such as, for example, 2,4-diamino-5-(3-methoxy-4,5-methylenedioxybenzyl)pyrimidine, prepared from the corresponding benzaldehydes, are described. The end products are useful as potentiators of the antibacterial activity of sulfonamides, as well as antibiotics.

Combination of oxytetracycline and

Compositions useful for conducting a plurality of different radioimmuno-assays simultaneously and methods for preparing same. The compositions radioimmunoassaya a plurality of labeled antigens and their respective antibodies present in selected concentrations so that the response curves observed for each antigen are essentially superimposable on the response curve of all other antigens present in the composition. Particularly preferred compositions include a mixture of radiolabeled morphine, a radiolabeled 5- or 5,5-substituted barbituric acid, i.e., secobarbital and their respective antibodies useful for performing a bivalent radioimmunoassay and a mixture of radiolabeled morphine, radiolabeled amphetamine, a radiolabeled 5- or 5,5-substituted barbituric acid and their respective antibodies useful for performing a trivalent radioimmunoassay.

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North Caldwell, NJ, United States of America

Emanuel Grunberg