Harvard College

Emanuel Carrilho

George M Whitesides

Katherine Andrea Mirica

Scott T Phillips

Andres W Martinez

Manish J Butte

Samuel W Thomas

Hayat Sindi

Sarah J Vella

Yanyan Liu

Amy Wong

Adam Carlyn Siegel

Sergey S Shevkoplyas

Adam Winkleman

Raquel Perez-Castillejos

Malancha Gupta

Benjamin Wiley

Phillip W Snyder

Katherine L Gudiksen

Andreas W Martinez

Sarah Jane Vella

Embodiments of the invention provide lateral flow and flow-through bioassay devices based on patterned porous media, methods of making same, and methods of using same. Under one aspect, an assay device includes a porous, hydrophilic medium; a fluid impervious barrier comprising polymerized photoresist, the barrier substantially permeating the thickness of the porous, hydrophilic medium and defining a boundary of an assay region within the porous, hydrophilic medium; and an assay reagent in the assay region.

Lateral flow and flow-through bioassay devices based on patterned porous media, methods of making same, and methods of using same

The ability to levitate, to separate, and to detect changes in density using diamagnetic particles suspended in solutions containing paramagnetic cations using an inhomogeneous magnetic field is described. The major advantages of this separation device are that: i) it is a simple apparatus that does not require electric power (a set of permanent magnets and gravity are sufficient for the diamagnetic separation and collection system to work); ii) it is compatible with simple optical detection (provided that transparent materials are used to fabricate the containers/channels where separation occurs; iii) it is simple to collect the separated particles for further processing; iv) it does not require magnetic labeling of the particles/materials; and v) it is small, portable. The method and kits provided provide for separation and collection of materials of different densities, diagnostics for detection of analytes of interest, monitoring of solid-supported chemical reactions and determination of densities of solid and liquid mixtures.

Density-based methods for separation of materials, monitoring of solid supported reactions and measuring densities of small liquid volumes and solids

Three-dimensional microfluidic devices including by a plurality of patterned porous, hydrophilic layers and a fluid-impermeable layer disposed between every two adjacent patterned porous, hydrophilic layers are described. Each patterned porous, hydrophilic layer has a fluid-impermeable barrier which substantially permeates the thickness of the porous, hydrophilic layer and defines boundaries of one or more hydrophilic regions within the patterned porous, hydrophilic layer. The fluid-impermeable layer has openings which are aligned with at least part of the hydrophilic region within at least one adjacent patterned porous, hydrophilic layer. Microfluidic assay device, microfluidic mixer, microfluidic flow control device are also described.

Three-dimensional microfluidic devices

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Chestnut Hill, MA, United States of America

Emanuel Carrilho