Monsanto Technology LLC

Elysia K Krieger

Edwards M Allen

Sara Elizabeth Heisel

Larry A Gilbertson

Shihshieh Huang

Yuanji Zhang

Sergey Ivashuta

Xudong Ye

Jennifer Lynn Lutke

Robert J Meister

Thomas M Malvar

Stanislaw Flasinski

James Kevin Roberts

Ervin D Nagy

Krishnakumar Sridharan

Farhad Moshiri

Barry S Goldman

Liang Guo

Anne-Marie Light Mettus

Wanggen Zhang

Barbara Isaac

Sakuntala Sivasupramanian

David K Kovalic

Jeffrey M Staub

Richard J Lawrence

Nancy M Houmard

Jianping Xu

Zarir E Vaghchhipawala

David K Kovalik

Sergey I Ivashuta

Yuanji I Zhang

Sara E Heisel

This disclosure is related to plant-optimized recombinant nucleic acids encoding Cpf1 and their use in planta. Also disclosed are compositions, expression cassettes, and plant cells comprising the recombinant nucleic acids as well as methods and kits for modifying a target sequence in a plant genome using the recombinant nucleic acids.

Compositions and methods for genome editing in planta

The present disclosure is in the field of plant transformation. The disclosure provides methods for increasing the rate of site-directed integration of a sequence of interest in plants. The disclosure also provides methods for increasing the efficiency of Rhizobiales-mediated plant transformation.

Methods and compositions for use in genome modification in plants

This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.

Regulation of gene expression by temporal or leaf specific promoters

The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

Recombinant DNA constructs and methods for controlling gene expression

This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.

Plant microRNAs and methods of use thereof

Temporal regulation of gene expression by microRNAs

Temporal regulation of gene expression by MicroRNAs

The present invention has incorporated a non-lethal negative selectable marker gene into the vector backbone DNA of a DNA plasmid used to transform plant cells. These transgenes are designed to express a non-lethal gene product in plant cells that contain the vector backbone DNA of the DNA plasmid. The gene products of the non-lethal negative selectable marker gene are involved in plant hormone biosynthesis pathways, plant hormone substrate diversion, plant hormone degradation, plant hormone signaling or metabolic interference. The use of these DNA plasmids to transform plant cells provides for the enhanced production of commercially viable plants.

DNA constructs and methods to enhance the production of commercially viable transgenic plants

Growing community of inventors

Kirkwood, MO, United States of America

Elysia K Krieger