University of Massachusetts

Elvan Cavac

Kithmie Harshana Malagodapathiranage

Sarah L Perry

Shuo Sui

Yasaman Gholamalipour

Craig Martin

Craig T Martin

Described herein are synthetic methods for producing sequence-specific RNA oligonucleotides that eliminate impurities produced in prior art methods. In one aspect, a first amplification primer includes one or more deoxyuridine residues, wherein at least one of the one or more deoxyuridine residues is at position −1, −2, −3, −4 or −5 of the promoter region for the single-subunit, DNA-dependent RNA polymerase. The deoxyuridines are excised to provide an amplified functional template DNA which is then used to synthesize RNA which has reduced immunogenic double stranded RNA compared to controls.

Enzymatic methods to generate high yields of sequence specific RNAs with extreme precision

Described herein are synthetic methods for producing sequence-specific RNA oligonucleotides that eliminate impurities produced in prior art methods. In one aspect, an end-protected capture DNA complementary to a portion of the product RNA is employed. In another aspect, the template DNA is covalently or noncovalently linked to the RNA polymerase, either directly or through the use of a nontemplate DNA. In a third aspect, a flow chamber is employed. All of the methods can be used in combination.

Enzymatic methods to generate high yields of sequence specific RNA oligonucleotides with extreme precision

Enzymatic methods to generate high yields of sequence specific rna with extreme precision

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Northampton, MA, United States of America

Elvan Cavac