The Charles Stark Draper Laboratory, Inc.

Else M Vedula

Joseph L Charest

Brett C Isenberg

Jeffrey T Borenstein

Jonathan Robert Coppeta

Abigail June Spencer

Corin Williams

Morgan Pilkenton

Elizabeth Ellen Marr

Patrick Davis

Ernest Kim Soonho

The systems and methods of the present disclosure provide highly deformable porous membrane culture systems and actuation methods for studying the effects of biomedical stretch on cultured tissue. A well plate can include a well having a first opening configured to receive an insert coupled to a deformable membrane. The well plate can include a gasket positioned within the well and configured to create a seal between the insert and the well when the insert is positioned in the well. The well plate can include a chamber defined beneath the well, the chamber configured to receive fluid media and to expose the fluid media to a surface of the deformable membrane when the insert is positioned in the well. The well plate can include an actuator configured to stretch the deformable membrane by a target amount of strain.

Highly deformable porous membrane culture system and actuation methods for studying the effects of biomechanical stretch on cultured tissue

The methods and systems described herein provide a cell culture platform with an array of tissue modeling environments and dynamic control of fluid flow. The cell culture platform includes an array of wells that are fluidically coupled by microchannel structures. The dynamically controlled flow of fluid interacts with cells grown within the microchannels.

Bi-layer multi-well cell culture platform

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Brookline, MA, United States of America

Else M Vedula