Plant Genetic Systems N.v.

Bayer Bioscience N.v.

Els Prinsen

Johan Botterman

Peter Ulvskov

Morten Petersen

Henri Van Onckelen

Bernhard Borkhardt

Robin Child

Lilli Sander

Gert Bundgard Poulsen

&ldquo;The present invention relates to DNA sequences, comprising nucleic acid fragments encoding dehiscence zone-selective proteins, particularly cell wall hydrolases such as polygalacturonases, and their use for modifying dehiscence properties in plants, more particularly pod dehiscence properties in

Seed shattering

A plant is provided which contains at least one dehiscence zone (DZ)-selective chimeric gene incorporated in the nuclear genome of its cells, wherein said DZ-selective chimeric gene comprises the following operably linked DNA fragments: a) a transcribed DNA region encoding: 1) a RNA which, when produced in cells of a particular DZ of the plant, prevents, inhibits or reduces the expression in DZ cells of an endogenous gene of the plant encoding a cell wall hydrolase, particularly an endopolygalacturonase, or, 2) a protein or polypeptide, which when produced in DZ cells, kills or disables them or interferes with their normal metabolism, physiology or development; b) a plant expressible promoter which directs expression of transcribed DNA region at least in DZ cells, provided that if transcribed DNA region encodes a protein or polypeptide, or encodes an antisense RNA or ribozyme directed to a sense RNA encoded by an endogenous gene that is expressed in plant in cells other than DZ cells, plant expressible promoter is a DZ-selective promoter, which directs expression of transcribed region selectively in DZ cells and wherein plant is characterized by modified dehiscence properties, preferably delayed dehiscence, when compared to a plant not containing DZ-selective chimeric gene.

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Kontich, Belgium

Els Prinsen