Aj Innuscreen Gmbh

Ist Innuscreen Gmbh

Moldiax Gmbh

Elmara Graser

Timo Hillebrand

Claus Knippschild

Benjamin Jaschinsky

The invention relates to a method for detecting various analytes, characterized by the following steps: a) providing separation particles containing, on their surface, firstly means of binding the analyte to be identified and secondly means of separating the analyte bound to the particles; b) providing identification particles firstly having, on their surface, means for binding the analyte to be identified and secondly containing on their surface or enclosed therein, means which are capable, after they have been detached or released from the particles, by virtue of their labeling, of generating a signal which serves for identification of the analyte; c) combining analyte, separation particles and identification particles; d) removing and washing the identification particles bound via the analyte by means of the separation particles; e) releasing the means which serve to identify the analyte, characterized in that the means which serve to identify the analyte are coupled reversibly to the identification particles and in that the identification molecules serve simultaneously for identification of the analyte and for detection.

Universal method for detecting various analytes

A method and test kit are useful for detecting specific nucleic acid sequences. The process includes (1) matrix-dependent new synthesis of the target nucleic acid; (2) target-specific probe hybridization; and (3) detection of the hybridization event. In the first step, an oligonucleotide 1, which is marked by a marker 1 and is entirely or partially complementary to the target sequence, acts as a primer in the matrix-dependent new synthesis of the target nucleic acid and, in the second step, an oligonucleotide 2, which is marked by a marker 2 and, owing to its melting temperature being lower than that of the oligonucleotide 1, is not involved in the first step, partially or completely hybridizes with the DNA new synthesis product of oligonucleotide 1.

Method for detecting specific nucleic acid sequences

A universally usable method for specific detection of target nucleic acid sequences, which method can be performed very rapidly and also simply and furthermore which does not need any expensive instrumental systems. The method is intended to be suitable as a molecular genetic rapid test and to respect the requirements of diagnostic specificity assurance. In this regard it is important that only one specific amplification product be detected and that amplification artifacts can be unambiguously discriminated. A nucleic acid amplification kit suitable for performing this method.

Method and rapid test for the detection of specific nucleic acid sequences

The invention relates to a device that allows a target nucleic acid to be detected in a homogeneous batch using two different detection formats. The device comprises at least two heatable sample blocks and a reaction cartridge which contains a base () and at least one film () sealing the base (), wherein the film () comprises a surface () that is not connected to the base () and said surface () forms a volume () for media transfer or at least one reaction chamber (). The device can be used in particular for mobile gene diagnostics under field conditions.

Device for detecting nucleic acids

A mobile rapid test system for nucleic acid analysis. A method comprising the steps of amplification of the nucleic acids by means of rapid-PCR technology, conversion of a double-stranded amplification product into a single-stranded DNA fragment, hybridization with a labeled probe and detection of the nucleic acids on a lateral-flow test strip. A device comprising a reaction cavity which preferably consists of a thin film, inlet and outlet openings for the reaction cavity, one or more heatable sample blocks which are connected to miniaturized cooling bodies and a window for reading off the result. The lateral-flow test strip is a component of the mobile rapid test system. Operation of the instrument system requires no external power source, but only batteries or a rechargeable battery.

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Berlin, Germany

Elmara Graser