10x Genomics, Inc.

Elliott Meer

Zachary Bent

Paul Ryvkin

Jessica Michele Terry

Serge Saxonov

Christopher Hindson

John R Stuelpnagel

Katherine Pfeiffer

Andrew D Price

Tarjei Sigurd Mikkelsen

Josephine Harada

Niranjan Srinivas

Christopher Joachim O'Keeffe

Solongo B Ziraldo

Phillip Belgrader

Geoffrey McDermott

Mohammad Rahimi Lenji

Vijay Kumar Sreenivasa Gopalan

Daniel P Riordan

Rajiv Bharadwaj

Michael Ybarra Lucero

Tobias Daniel Wheeler

Indira Wu

Stephane Claude Boutet

Sarah Taylor

Alex Gagnon

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for 'tagging' analytes for characterization.

Methods and systems for processing polynucleotides

The present disclosure provides methods of generating supports (e.g., beads) comprising barcode molecules coupled thereto. A barcode molecule coupled to a support may comprise a barcode sequence and a functional sequence. A barcode molecule may be generated using two or more ligation reactions in a combinatorial fashion. A support comprising two or more different barcode molecules may be useful for analyzing or processing one or more analytes such as nucleic acid molecules, proteins, and/or perturbation agents.

Functionalized gel beads

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

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Pleasanton, CA, United States of America

Elliott Meer