The United States of America As Represented by the Secretary of the Department of the Interior

Elliott Barnhart

Alfred B Cunningham

Robert Hyatt

Mathew Fields

A Sampler and process for collecting, growing, inoculating and evaluating microbes in-situ. The Sampler may be loaded with an active or inert media to assist with the collection of microbes. It is closed and lowered to a desired depth. Sampler is opened to initiate sampling. Materials are added to the sampler to stimulate growth or change the environment. To collect and grow in situ microbes, the sampler is left open for a period of time, a trigger closes and seals the sampler capturing contents specific to that location. The sampler is lifted preserving the in-situ conditions from the collection location. The contents of the sampler can be interrogated through sealed ports at the field site or at a remote location. The sampler can also be utilized as a biologic reactor allowing researchers to connect laboratory equipment to analyze, modify, or cultivate the collected sample.

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Clancy, MT, United States of America

Elliott Barnhart