Thomas Jefferson University

The Wistar Institute

University of Pennsylvania

Drexel University

Elliot M Levine

Bruce E Jarrell

Sandor S Shapiro

Cato T Laurencin

Solomon R Pollack

Helen H Lu

Edward Botchwey

Mohammed Yusuf Khan

Scaffolds for tissue engineering prepared from biocompatible, biodegradable polymer-based, lighter than or light as water microcarriers and designed for cell culturing in vitro in a rotating bioreactor are provided. Methods for preparation and use of these scaffolds as tissue engineering devices are also provided.

Biocompatible, biodegradable polymer-based, lighter than or light as water scaffolds for tissue engineering and methods for preparation and use thereof

Vigorous growth of endothelial cells from human blood vessels in vitro is achieved using a culture medium containing endothelial cell growth factor and heparin and/or dextran sulfate. Cell population doubling times of 13 to 21 hours for 60 to 85 populations doublings are obtained, and cloned human endothelial cell strains are established having similar proliferative capacities.

Process and medium for cloning and long-term serial cultivation of adult

Vigorous growth of endothelial cells from human umbilical vein endothelial cells in vitro is achieved using a culture medium containing endothelial cell growth factor and heparin and/or dextran sulfate. Cell population doubling times of 13 to 21 hours for 60 to 85 populations doublings are obtained, and cloned human endothelial cell strains are established having similar proliferative capacities.

Process and medium for cloning and long-term serial cultivation of human

Growing community of inventors

Cherry Hill, NJ, United States of America

Elliot M Levine