Roche Molecular Systems, Inc.

Other

Bayer Corporation

Roche Diagnostics Gmbh

F. Hoffmann-La Roche Ag

Ellen H Fiss

Thomas W Myers

Jingtao Sun

David H Gelfand

Keith Bauer

Stephen Gordon Will

Shawn Lee Suko

Edward Soh Smith

Slav Dugenny

Eugene G Spier

Veeraiah Bodepudi

Dirk Zimmermann

Joseph San Filippo

Nicolas Newton

Joachim Glaubitz

Meike Eickhoff

Rachel Shahinian

Marintha Heil

Niclas Hitziger

Jeffery Fong

Brian David Warner

Thomas Horn

Say-Jong Law

Nancy J Schoenbrunner

Todd B Sells

Susanne Stoffel

Hartmut Schroeder

Marc Rehfuss

Edward S Smith

Olga Budker

Thomas William Myers

Keith A Bauer

Shawn Suko

Eugene Spier

David Harrow Gelfand

The present invention provides inactivated microbes, methods of preparing and using the same, as well as compositions and kits containing the same. The inactivated microbes are useful in the formulation of internal control reagents for use in recombinant nucleic acid techniques, especially nucleic acid amplification, e.g., by the polymerase chain reaction (PCR).

Method of inactivating microbes by citraconic anhydride

Methods for the rapid detection of the presence or absence ofin a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targetingand kits are provided that are designed for the detection of, including, but not limited to, thespecies of, and. Also described are kits, reaction mixtures, and oligonucleotides (e.g., primer and probe) for the amplification and detection of

Compositions and methods for detection of

Methods for the rapid detection of the presence or absence of Zika virus in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting Zika virus and kits are provided that are designed for the detection of Zika virus.

Compositions and methods for detection of zika virus

Methods and systems are provided for packaging reporter nucleic acid molecules into non-replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non-replicative transduction particles as reporter molecules.

Non-replicative transduction particles and transduction particle-based reporter systems

The invention comprises a reversible oligonucleotide inhibitor of nucleic acid polymerases. Methods of designing said inhibitors and using said inhibitors in amplification and detection of nucleic acid, particularly detection of RNA by RT-PCR are also disclosed.

Oligonucleotide inhibitor of DNA polymerases

Compositions and methods for detection of Zika virus

Methods and oligonucleotides are provided for detecting an internal control nucleic acid for qualitative and/or quantitative purposes.

Oligonucleotides for controlling amplification of nucleic acids

Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

DNA polymerases and related methods

Alkyl amines act to release formaldehyde cross-linking that occurs in biological samples. Thus, contacting alkyl amines to formaldehyde fixed samples is a useful way to render biological components of the samples, including nucleic acids or proteins, more accessible to detection and characterization.

Alkyl amines improve detection of components of formaldehyde-fixed biological samples

Growing community of inventors

Albany, CA, United States of America

Ellen H Fiss