Max-Planck-Gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Haemosys Gmbh

Jenaffin Gmbh

Senova Gesellschaft Fur Biowissenschaft Und Technik Mbh

Elke Bucha

Gotz Nowak

Götz Nowak

Ute Lange

Hans-Jurgen Kolde

Verena Baldinger

Jutta Hoffman

Hans-Juergen Kolde

Hans-Jürgen Kolde

The present invention is directed to a simple process for the modification of the specificity of a peptidase of the hemostatic system by coupling polymers to the peptidase causing it to lose its reactivity in the hemostatic system, but enabling it to continue to react with certain inhibitors, effectors and substrates. The invention is furthermore directed to processes for the detection or quantitative determination of inhibitors of the peptidase in bodily fluids or other samples as well as to processes for their neutralization and/or removal from liquids. Finally, the invention allows the use of a polymer-coupled peptidase as drug and furthermore provides a device which makes use of such a peptidase in the removal of peptidase inhibitors from samples or from the bloodstream of a patient.

Polymer-coupled peptidases

The invention relates to a method for determining the total clotting activity of a blood or plasma sample, characterized in that a highly specific, reversible thrombin inhibitor is added to a blood or plasma sample in a defined amount, the clotting of the blood or plasma sample is induced and, after a defined period of time, the consumed amount of the added thrombin inhibitor is determined in a per se known manner, as well as to a kit for obtaining information about the state of coagulation of a blood sample.

Method for determining the total clotting activity of a blood or plasma sample

The invention relates to a method for determining the concentration of thrombin inhibitors in a non-turbid body liquid or a non-turbid extract from a body liquid. It comprises the following steps. The body liquid is taken from a living body, and the body liquid is subjected to a separation from the turbid matter, if necessary. To the non-turbid body liquid thus obtained are added a coagulation-inhibiting substance not interfering in the transformation prothrombin/active meizothrombin or Mtdesfg1, resp., a chromogenic or fluorogenic substrate not dissociable by active meizothrombin or Mtdesfg1, resp., and a substance dissociating prothrombin into meizothrombin or Mtdesfg1, resp., and as an option prothrombin. The thus obtained solution or mixture, resp., is subjected to a wavelength-selective light absorption or light emission measurement as a function of the time. From the reduction of the light absorption or light emission per time unit is determined the amount of the thrombin inhibitor included in the body liquid by comparison to previously determined standard curves.

Method for determining the concentration of thrombin inhibitors

An interactive system is provided with at least one active surface of plastic from monomers containing at least one structural element derived from a carbon dioxide (A), and at least one substance associated to a linker with at least one structural element (B) capable of establishing a hydrogen bond, and involving an interaction between the structural elements (A) and (B). The interactive system is suitable for presenting and eliminating substances in liquids.

Interactive system for presenting and eliminating substances

The invention relates to measuring of the adhesion of platelets ex vivo. In this method, monodisperse polymer particles are added to blood, and the number of platelets in the blood are measured before and after shaking of the sample. The level of adhesion of the platelets is determined by comparing the number of platelets prior to shaking with the number of platelets after shaking. Methods of establishing an adhesion index and diagnosis of a patient are also provided.

Simple method for quantitative measuring the adhesion of platelets ex vivo

The invention relates to a blood-compatible surface comprising a polymer surface and a plurality of conjugates made of linkers and active agents immobilized thereon. The polymer surface contains similar or different structural units that carry carbonyl groups. The linkers contain a structural element that is able to form a hydrogen bridge bond. A polyorganosiloxane acting as the active agent is linked to the linkers.

Blood compatible polymer surfaces

The invention relates to a method for determining the concentration of thrombin inhibitors in a non-turbid body liquid or a non-turbid extract from a body liquid. It comprises the following steps. The body liquid is taken from a living body, and the body liquid is subjected to a separation from the turbid matter, if necessary. To the non-turbid body liquid thus obtained are added a coagulation-inhibiting substance not interfering in the transformation prothrombin/active meizothrombin or Mtdesfgl, resp., a chromogenic or fluorogenic substrate not dissociable by active meizothrombin or Mtdesfgl, resp., and a substance dissociating prothrombin into meizothrombin or Mtdesfgl, resp., and as an optional prothrobmin. The thus obtained solution or mixture, resp., is subjected to a wavelength-selective light absorption or light emission measurement as a function of the time. From the reduction of the light absorption or light emission per time unit is determined the amount of the thrombin inhibitor included in the body liquid by comparison to previously determined standard curves.

Method for determining the concentration of thrombin inhibitors and kits therefor

The present invention relates to an interactive system comprising at least one active surface of plastic from monomers containing at least one structural element derived from a carbon dioxide (A), and at least one substance associated to a linker with at least one structural element (B) capable of establishing a hydrogen bond, and involving an interaction between the structural elements (A) and (B). That interactive system is suitable for presenting and eliminating substances in liquids.

The present invention relates to a process for separating substances, which are bonded to a polymer surface via a linker, by adding a polar organic solvent.

Separation process

Complex-bound inhibitors of metabolic enzymes capable of being

The invention relates to an antidote for hirudin and synthetic thrombin inhibitors; the antidote contains a compound that splits prothrombin into meizothrombin, a prothrombin intermediate, a pharmacologically acceptable salt thereof or a mixture of these compounds, together with conventional vehicles and/or diluents. The present invention also relates to the application of a compound that splits prothrombin into meizothrombin, a prothrombin intermediate, or a pharmacologically acceptable salt thereof or a mixture of these compounds, together with conventional vehicles and/or diluents as an antidote for hirudin and synthetic thrombin inhibitors or for the preparation of an antidote for hirudin and synthetic thrombin inhibitors.

Growing community of inventors

Erfurt, Germany

Elke Bucha