Leland Stanford Junior University

Novome Biotechnolooies, Inc.

Novome Biotechnologies, Inc.

Elizabeth Stanley

Justin L Sonnenburg

Weston R Whitaker

William C DeLoache

Provided are nucleic acids that include a promoter, where the promoter is operable in acell and is operably linked to a heterologous nucleotide sequence of interest. Also provided are nucleic acids that include a promoter (operable in a prokaryotic cell such as acell) operably linked to a sequence encoding a synthetic ribosomal binding site (RBS). Also provided are fusion proteins (and nucleic acids encoding them) in which a secretedpolypeptide is fused to a heterologous polypeptide of interest. Also provided are prokaryotic cells (e.g.,, acell, and the like) that include one more nucleic acids such as those described above. Also provided are methods of expression in a prokaryotic cell, methods of detectably labeling acell in an animal's gut, and methods of delivering a protein to an individual's gut.

Compositions and methods for nucleic acid expression and protein secretion in Bacteroides

Compositions and methods are provided for modulating growth of a genetically modified bacterial cell present in a human organ, for modulating growth of a genetically modified bacterial cell in an organ (e.g., gut), for displacing at least a portion of a population of bacterial cells in an organ, and for facilitating gut colonization by a genetically modified bacterial cell. Also provided are genetically modified bacterial cells, e.g., cells that include a heterologous carbohydrate-utilization gene or gene set that provides for the ability to utilize as a carbon source a rare carbohydrate of interest that is utilized as a carbon source by less than 50% of bacterial cells present in a human microbiome.

Growing community of inventors

Portola Valley, CA, United States of America

Elizabeth Stanley