General Electric Company

Leica Microsystems Cms Gmbh

Elizabeth Mary McDonough

Arunkumar Natarajan

Anup Sood

Lakshmi Sireesha Kaanumalle

Kevin Bernard Kenny

Dmitry Vladimirovich Dylov

The invention discloses a method for reduction of autofluorescence in biological samples, comprising the steps of:

Method for reduction of autofluorescence from biological samples

Methods comprising probing multiple targets in an H&E stained biological sample are provided. The methods include the steps of providing a hematoxylin and eosin stained biological sample containing multiple targets, optionally detecting H&E staining of the sample, removing the hematoxylin and eosin signals, and detecting additional features or targets in the biological sample. The detecting step may include performing the steps of binding at least one probe to one or more targets to the sample, detecting a signal from the probe and contacting the sample with a bleaching agent. The process of binding, detecting and bleaching may be iteratively repeated.

Methods of analyzing an H and E stained biological sample

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Niskayuna, NY, United States of America

Elizabeth Mary McDonough