Oxford Nanopore Technologies Limited

Vib Vzw

Vrije Universiteit Brussel

Katholieke Universiteit Leuven

Katholieke Universiteit Leuven, K.u. Leuven Research & Development

Elizabeth Jayne Wallace

Lakmal Nishantha Jayasinghe

Andrew John Heron

Mark Bruce

James Anthony Clarke

Richard George Hambley

James White

Szabolcs Soeroes

Rebecca Victoria Bowen

Jonathan Bankes Pugh

Ruth Moysey

Han Karel Remaut

John Milton

Clive Gavin Brown

Luke McNeill

Pratik Raj Singh

Christopher Peter Youd

Domenico Caprotti

Neil Roger Wood

David Alves

Michael Robert Jordan

Joseph Hargreaves Lloyd

Stefan Howorka

John Joseph Kilgour

Sander Egbert Van Der Verren

Nani Van Gerven

Luke Mcneill

Lakmal Jayasinghe

Giovanni Maglia

Mihaela Misca

Lorenzo Franceschini

Tine Brouns

Antonino Puglisi

Anthony Clarke

Mark John Bruce

David Antoni Alves

Han Remaut

The invention relates to mutant forms of CsgG. The invention also related to analyte detection and characterisation using CsgG.

Mutant CsgG pores

The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Dda helicase. The helicase controls the movement of the target polynucleotide through the pore. The invention also relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

Modified helicases

The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using lysenin.

Mutant lysenin pores

The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.

Mutant pores

The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.

Protein pores

Provided is a method of characterising a polynucleotide using a transmembrane pore, wherein the pore is a double pore comprising a first Csg G pore, or a homologue thereof, and a second CsgG pore, or a homologue thereof.

Transmembrane pore consisting of two CsgG pores

The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterization using CsgG.

Mutant pore

The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

Modified enzymes

Provided herein relate to modified or mutant forms of secretin and compositions comprising the same. In particular, the modified or mutant forms of secretin permits efficient capture and/or translocation of an analyte through the modified or mutant secretin nanopores. Methods for using unmodified secretin or the modified or mutant forms of secretin and compositions, for example, for characterizing an analyte, e.g., a target polynucleotide, are also provided.

Modified nanopores, compositions comprising the same, and uses thereof

The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein. The invention also relates to improved transmembrane pores and polynucleotide binding proteins.

Method of improving the movement of a target polynucleotide with respect to a transmembrane pore

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Oxford, United Kingdom

Elizabeth Jayne Wallace