Mogen International Nv

Rijksuniversiteit Te Leiden

Elisabeth J Meulenhoff

Leo Sjoerd Melchers

Bernardus Johannes Cornelissen

Charles Peter Woloshuk

Marianne Beatrix Sela-Buurlage

Petrus Josephus Van Den Elzen

John Ferdinand Bol

Hubertus J Linthorst

Jeroen S Van Roekel

Alexandra Aleida Vloemans

Plants are provided with improved resistance against pathogenic fungi. They are genetically transformed with one or more polynucleotides which essentially comprise one or more genes encoding plant chitinases and .beta.-1,3-glucanases. Preferred are the intracellular forms of the said hydrolytic enzymes, especially preferred are those forms which are targeted to the apoplastic space of the plant by virtue of the modification of the genes encoding the said enzymes. Particularly preferred are plants exhibiting a relative overexpression of at least one gene encoding a chitinase and one gene encoding a .beta.-1,3-glucanase.

Transgenic fungal resistant plants expressing chitinase and glucanase,

A method for inhibiting the growth and/or germination of a fungus that is susceptible to an osmotin protein by contacting the fungus, or causing the fungus to be contacted with, an effective amount of an osmotin protein. The fungus can be, for example, a plant pathogenic fungus, such as an Oomycete. In one embodiment of the invention, the effective amount of the osmotin protein is produced by a plant having incorporated into its genome a chimeric gene having a) an open reading frame encoding the osmotin protein, or a precursor of the osmotin protein, and having operably linked thereto b) a regulatory region which causes the chimeric gene to be expressed in the plant.

Antifungal preparations containing osmotin

Fungal resistant plants, process for obtaining fungal resistant plants

Antifungal preparations, and process for making such preparations

Growing community of inventors

Amsterdam, Netherlands

Elisabeth J Meulenhoff