Ge Healthcare Bio-sciences Ab

Ge Healthcare Bioprocess R&d Ab

Elisabeth Hallgren

Klaus Gebauer

Ronnie Palmgren

Ola Lind

Kajsa Stridsberg-Friden

Jamil Shanagar

Raf Lemmens

Bjorn Johan Lundgren

Helena Widegren

Linda Svensson

Björn Lundgren

Bjoern Lundgren

Kajsa Stridsberg-FRIDÉN

The invention provides a packing method for high efficiency chromatography columns starting from dry swellable particles, as well as columns packed by the method and the use of the columns in separation of biomolecules. In the packing method, an amount of dry swellable particles sufficient to give a swollen volume in a liquid of about 105-120% of the column chamber volume is transferred to the column, the column is closed and the liquid is provided to the column.

Method for dry packing chromatography columns

The present invention relates to a method of separating a target from a liquid, which includes providing a polymer in an aqueous liquid, which polymer comprises at least one hydrophobic portion; contacting the polymer-containing liquid with the liquid comprising the target; applying a stimulus to the resulting mixture; and maintaining it until a reversible phase separation is obtained. One phase is polymer-rich and contains target(s) and another phase is polymer-poor. By either maintaining the stimulus, or applying a different stimulus and maintaining it, the polymer-rich phase is transformed into a substantially solid phase.

Preparation of biomolecules

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Uppsala, Sweden

Elisabeth Hallgren