Speedx Pty Ltd

Janssen R&d Ireland

Speeddx Pty Ltd

Janssen Sciences Ireland Uc

Tibotec Pharmaceuticals Ltd.

Johnson & Johnson Research Pty Limited

Janssen Diagnostics Bvba

Elisa Mokany

Alison Velyian Todd

Dina Lonergan

Evelyn Meiria Linardy

Cortny Donald

Lit Yeen Tan

Tram Bich Doan

Samantha Walker

Donald John Birkett

Paul Ean Young

Lieven Jozef Stuyver

Marie-Pierre T M M G De Bethune

Guenter Kraus

David Nauwelaers

Laurence Tatiana Rimsky

Christopher Roland Reid

Bart Anna Julien Maes

Inky Paul Madeleine De Baere

Marie-Pierre Tmmg De Bethune

Bart Anna Maes

Inky Paul De Baere

Helen Anne Swain

Marie-Pierre TMMG De Bethune

The present invention provides modified oligonucleotides and methods for their use in the detection of nucleic acids. The oligonucleotides and methods find particular application in amplifying and/or detecting areas of genetic variation in target nucleic acid sequences.

Detection of nucleic acids

The invention relates to nucleic acid substrates for catalytic nucleic acid enzymes and methods utilizing the substrates.

Nucleic acid enzyme substrates

The present invention relates to compositions and methods for the use of enzymes composed of nucleic acid and/or protein enzymes to generate and amplify a signal indicative of the presence of a target. More particularly, the invention relates to compositions comprising nucleic acid structures that serve as partial or complete enzyme substrates and methods for using these structures to facilitate detection of targets.

Signal amplification

The present invention relates to compositions and methods that allow the manipulation of the catalytic activity of multi-component nucleic acid complexes. Further, the invention provides methods which use these compositions and methods to create molecular sensors, molecular switches, and/or modulators or propagators of autocatalytic self-replicating cascades and other iterative processes. More particularly, the invention relates to compositions allowing self-assembly of active and inactive multicomponent nucleic acid complexes, methods of making such compositions, and methods for use.

Molecular switches and methods for their use

The present invention relates to methods that utilize multi-component nucleic acid complex (MNA complex) cascades. The MNA complexes may have cleavage or ligase activity. Further, the invention provides cascades which may include one or more DNAzymes. The invention also provides methods which use these cascades for the identification, detection and quantification of targets.

Nucleic acid enzymes and complexes and methods for their use

The present invention relates to Multicomponent Nucleic Acid Enzymes (MNAzymes) and methods for their use. MNAzymes comprise two or more oligonucleotide components which self-assemble in the presence of one or more MNAzyme assembly facilitator molecules to form a catalytically active structure. Compositions for making MNAzymes, and collections of MNAzymes are provided. Also provided are methods for using MNAzymes for the detection, identification and/or quantification of one or more targets. The methods can be practiced in solution-based assays or in assays where one or more reaction components are attached to a support structure. The methods allow for multiplexing the MNAzyme detection to detect multiple targets in a single reaction. Also provided are kits for making the compositions, and for practicing the methods provided herein.

Multicomponent nucleic acid enzymes and methods for their use

The present invention relates to methods and means for the evaluation of HIV replicative capacity in a given environment. In particular, the invention provides a growth competition assay that can determine relative viral fitness using a recombinant tagged HIV-1 virus system. The methods rely on plasmid vectors, amplicons, primers and probes, and the generation of replication-competent viruses therefrom. Said methods and materials may find use in multiple fields including diagnostics, drug screening, pharmacogenetics and drug development.

Methods, plasmid vectors and primers for assessing HIV viral fitness

The invention concerns a method for the extraction of nucleic acids from biological samples e.g. tissue material or sputum derived from human or animal species and the quantitative detection thereafter of said nucleic acids e.g. in terms of viral load, more specifically RSV viral load detection.

Respiratory syncytial virus (RSV) viral load detection assay

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Caringbah, Australia

Elisa Mokany