University of California

The Brigham and Women's Hospital, Inc.

Harvard College

The Broad Institute, Inc.

Elisa Franco

Amit Choudhary

Kurt Cox

Peng Wu

Hari Subramanian

Basudeb Maji

Hari K K Subramanian

Compositions and methods for conditionally regulating activities of CRISPR-Cas systems. In some embodiments, the methods comprise providing an inactive guide RNA comprising a regulatory domain bound by a lock nucleic acid different from the guide RNA; and displacing the lock nuclei acid from the regulatory domain by a trigger nucleic acid, thereby activating the guide RNA, wherein the activated guide RNA forms a complex with a Cas enzyme.

Compositions and methods for regulating proteins and nucleic acids activities

Compositions and methods are provided for the inhibition of the function of RNA guided endonucleases, including the identification and use of such inhibitors.

Inhibitors of RNA guided nucleases and uses thereof

The embodiments disclosed herein utilize fluorescence polarization based preliminary screen to identify a putative set of Cas inhibitors from an initial set of candidate inhibitors. The primary screening assay is followed by secondary screening assay to validate the putative set of inhibitors selected by the preliminary screen. In some embodiments, the present disclosure includes compositions and methods are provided for the inhibition of the function of RNA guided endonucleases, including the identification and use of such inhibitors.

CRISPR protein inhibitors

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Oakland, CA, United States of America

Elisa Franco