The Charles Stark Draper Laboratory, Inc.

The General Hospital Corporation

The Brigham and Women's Hospital, Inc.

Massachusetts Institute of Technology

Other

Eli J Weinberg

Jeffrey T Borenstein

Joseph P Vacanti

Mohammad Reza Kaazempur-Mofrad

Peter Mack

Guillermo Garcia-Cardena

Ahmad S Khalil

Kevin R King

Jennifer Ogilvie

Alexander Sevy

Tomoyuki Maemura

Osamu Ishii

Chiao-Chun Wang

Young-Moon Michael Shin

Ezra D Eskandry

Malinda M Tupper

Sarah L Tao

Jason Oliver Fiering

Ernest Soonho Kim

James Hsiao

William J Adams, Jr

Theodore Marentis

Nicholas J Krebs

Edward J Barnard

Eleanor M Pritchard

Brian K Orrick

Stephen L Bellio

Mitchell L Hansberry

James Ching-Ming Hsiao

Sarah Tao

William James Adams

Microfluidic structures featuring substantially circular channels may be fabricated by embossing polymer sheets.

Microfluidic structures for biomedical applications

Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick, structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.

Fabrication of vascularized tissue using microfabricated two-dimensional molds

The systems and methods described herein relate to a high-throughput flow apparatus. The apparatus is used with an array of wells, and is configured to impart a predetermined shear stress on cells cultured within each of the wells of the array of wells. The apparatus includes a plurality of mechanical tips. The plurality of mechanical tips each includes a head with a hemispheroid shape. The apparatus also includes a motor associated with at least one of plurality of mechanical tips. The motor is configured to drive the plurality of mechanical tips to impart the shear stress pattern in each of the wells.

Systems and method for high-throughput testing

microfluidic structures for biomedical applications

A high-throughput flow system includes an array of wells and a separate mechanical tip positioned within each well. Each mechanical tip is separately actuated to impart a shear stress pattern. A separate sleeve may be associated with each tip for maintaining a predetermined distance between the tip and a floor of the tip's corresponding well, with each tip being rotatable within its corresponding sleeve. Alternatively, a separate post may be associated with each tip for maintaining a predetermined distance between the tip and a floor of the tip's corresponding well, with each tip being rotatable about its corresponding post.

High-throughput biological screening

Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.

Methods and materials for making an apparatus which duplicates the functionality of a physiological system is provided.

Micromachined bilayer unit of engineered tissues

The present invention relates to bioartificial devices and systems that mimic kidney or nephron function and methods of making them.

Systems, methods and devices relating to a cellularized nephron unit

The present invention relates to a three-dimensional system, and compositions obtained therefrom, wherein individual layers of the system comprise channels divided longitudinally into two compartments by a centrally positioned membrane, and wherein each compartment can comprise a different cell type.

Microfabricated compositions and processes for engineering tissues containing multiple cell types

Methods of fabricating microfluidic structures featuring substantially circular channels are provided. The methods involve (a) providing a patterned wafer comprising at least one exposed electrically conductive region and at least one exposed electrically insulating region; (b) electroplating an inverse channel portion with substantially semicircular cross section onto the wafer, thereby forming a first master mold; (c) employing the first master mold so as to emboss a channel portion in a first polymer sheet; and (d) aligning and bonding the first polymer sheet with a second polymer sheet having a corresponding channel portion such as to define a first channel with substantially circular cross section between the polymer sheets.

Method of fabricating microfluidic structures for biomedical applications

The present invention relates to methods for the design and fabrication of biological constructs, such as organ simulants or organ replacements, which contain complex microfluidic architecture. Designs of the present invention provide increased space in the lateral dimension, enabling a large number of small channels for small vessels.

Three dimensional construct for the design and fabrication of physiological fluidic networks

Systems, methods, and devices relating to a cellularized nephron unit

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Needham, MA, United States of America

Eli J Weinberg