Aarhus Universitet

Plant Bioscience Limited

Elena Simona Radutoiu

Esben Bjørn Madsen

Lene Heegaard Madsen

Jens Stougaard Jensen

Zoltan Bozsoki

Kasper Røjkjær Andersen

Esben Bjorn Madsen

Kira Gysel

Simon Boje Hansen

Leila Tirichine

Niels Norgaard Sandel

Lene H Madsen

Niels Nørgaard Sandal

Jens Stougaard

Aspects of the present disclosure relates to genetically altered LysM receptors. In particular, the present disclosure relates to a hydrophobic patch into the LysM2 domain which can increase affinity and/or selectivity for LCOs and by replacement of regions in the LysM1 domain with the corresponding regions of the LysM1 domain from a donor LysM receptor that can alter the affinity and/or selectivity for the oligosaccharide particularly for LCOs and can alter the specificity between LCO when using regions from a high affinity and specificity LCO LysM receptor such as a legume NFR1 receptor. The present disclosure also relates to genetically altering LysM receptors in plants to include a hydrophobic patch or alter the hydrophobic patch and to genetically altering LysM receptors in plants by replacement of regions in the LysM2 domain.

Genetically altered LysM receptors with altered agonist specificity and affinity

The present invention provides a Nod-factor binding element, comprising one or more NFR polypeptides encoded by NFR genes, that are useful for providing non-nodulating plants with Nod-factor binding properties and triggering the endosymbiotic signalling pathway leading to nodulation. Furthermore the invention is useful for breeding for improved nodulation in nodulating legumes.

Nod-factor perception

Growing community of inventors

Hojbjerg, Denmark

Elena Simona Radutoiu