The University of Chicago

Other

University of Virginia Patent Foundation

Cellscript, LLC

Elena K Davydova

Lucia B Rothman-Denes

Krystyna Maria Kazmierczak

Jerome J Jendrisak

Gary A Dahl

Anthony A Kossiakoff

Zygmunt S Derewenda

Mateusz Jaskolowski

Yuri Saveliev

Svetalana Y Gerdes

Daniel A Engel

Helmut Fluhrer

This disclosure is directed to compositions and methods for utilizing the boundaries of the C-terminal domains of Nucleoprotein from Zaire Ebola virus as highly stable recombinant protein antigens to generate antibodies for diagnosis and treatment of Ebola virus infection.

Recombinant antibodies that recognize the C-terminal domains of Ebola virus nucleoprotein

The present invention provides methods, compositions and kits for using an RNA polymerase for making transcription products corresponding to a target sequence by obtaining single-stranded DNA transcription substrates that comprise a single-stranded promoter that is operably joined to a single-stranded target sequence. The invention has broad applicability for research, diagnostic and therapeutic applications, such as preparing cDNA corresponding to full-length mRNA, making sense or anti-sense probes, detecting gene- or organism-specific sequences, cloning, cell signaling or making RNA for use in RNAi.

Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences

An apparatus for modification of the atmospheric humidity profile via creating updrafts of moistened air is provided. The apparatus is based on a capacitor of a Van der Graaf generator which, with the aid of air ionizers comprising a source of alpha particles, produces a unipolar atmospheric electric current of a magnitude sufficient to achieve selective moisture transport. Operating such an apparatus augments or creates a moisture updraft, which may lead to the formation of new clouds and/or an increase in supersaturation in existing clouds, thus enhancing the development of precipitation and/or facilitating the inflow of the evaporated moisture from a water reservoir inland.

Apparatus for controlling atmospheric humidity

The present invention comprises novel methods, compositions and kits that use N4 vRNAP deletion mutants to detect and quantify analytes comprising one or multiple target nucleic acid sequences, including target sequences that differ by as little as one nucleotide or non-nucleic acid analytes, by detecting a target sequence tag that is joined to an analyte-binding substance. The method consists of an annealing process, a DNA ligation process, an optional DNA polymerase extension process, a transcription process, and, optionally, a detection process.

Target-dependent transcription using deletion mutants of N4 RNA polymerase

A histidine-tagged, deletion mutant of bacteriophage N4-coded, virion RNA polymerase (mini-vRNAP) which is active has been developed. The his-tagged mini-vRNAP has been cloned under the control of the pBAD promoter, is stable and is purified in a single step yielding large amounts (10 mg/liter ofexpressing cells). This RNA polymerase uses single-stranded DNA containing 17 bases (the promoter) upstream of the transcribed regions as a template. In the presence ofSSB protein, it transcribes this template efficiently, providing a unique system to synthesize RNAs of the desired sequence using single-stranded DNA templates. The enzyme incorporates derivatized nucleoside triphosphates with high efficiency. A mutant of mini-vRNAP has been generated that incorporates deoxynucleoside triphosphates. In addition, the inventors have developed an in vivo system to express RNAs and proteins under mini vRNA polymerase promoter control.

N4 virion single-stranded DNA dependent RNA polymerase

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Chicago, IL, United States of America

Elena K Davydova